Dose- and time-response curves were combined to assess the potential of the comet assay in radiation biodosimetry. The neutral comet assay was used to detect DNA double-strand breaks in lymphocytes caused by γ-ray irradiation. A clear dose-response relationship with DNA double-strand breaks using the comet assay was found at different times after irradiation (p < 0.001). A time-response relatio...

For risk assessment, it is necessary to evaluate the dose of human exposure to radiation. Ionizing radiation being an environmental genotoxic factor induces dosedependent somatic mutations. Recent studies support the somatic-mutation theory of carcinogenesis. Mutations at the Glycophorin–A (GPA) loci have been studied for establishment of somatic mutation assays. GPA assay provides a lifetime b...

Cytogenetic dosimetry plays an important role in the triage and medical management of affected people in radiological incidents/accidents. Cytogenetic biodosimetry uses different methods to estimate the absorbed dose in the exposed individuals, and each approach has its advantages and disadvantages. Premature chromosome condensation (PCC) assay presents several advantages that hopefully fulfill...

In the modern developing society, application of radiation has increased extensively. With significant improvement in protection practices, exposure to human could be minimized substantially, but cannot avoided completely. Assessment is essential for regulatory decision and medical management as applicable. Until now, cytogenetic changes have served surrogate marker been extensively employed bi...

Introduction DNA damage is among the main consequences of radiation. Of many different classes of DNA damage, double-strand breaks are the most deleterious. Development of a sensitive biodosimetry method, which utilizes a detection material with a similar construction to the body, seems essential for monitoring radiation workers. In this study, histone H2AX protein was examined as a potential  ...