Introduction: The chronic and recalcitrant nature of rhinosinusitis has been known from many years. Many reasons for this have been implicated and biofilms have now been established as one of the cause for its recurrent and persistent nature. Little literature and studies exist confirming this effect. This study presents analysis of sinonasal mucosal samples and correlates presence of biofilms ...

background chlorhexidine gluconate (chx) is the most common antimicrobial agent used against oral pathogens, however information on its ability to inhibit and disrupt anaerobic single and multispecies biofilms is relatively unknown. objectives the purpose of this study was to test the efficacy of chx for its biofilm inhibition and disruption properties using crystal violet assay. materials and ...

objectives:biofilms are communities of bacteria attached to surfaces through an external polymeric substances matrix. in the meantime, acinetobacterbaumannii is the predominant species related to nosocomial infections. in the present study, the effect of silver nanoparticles alone and in combination with biocides and imipenem against planktonic and biofilms of a. baumannii was assessed.  materi...

Biofilms are communities of bacteria that exhibit a multitude of multiscale biomechanical behaviors. Recent experimental advances have lead to characterizations of these behaviors in terms of measurements of the viscoelastic moduli of biofilms grown in bioreactors and the fracture and fragmentation properties of biofilms. These properties are macroscale features of biofilms; however, a previous...

Using microscopic and molecular techniques combined with computational analysis, this study examined the structure and composition of microbial communities in biofilms that formed on different artificial substrates in a brine pool and on a seep vent of a cold seep in the Red Sea to test our hypothesis that initiation of the biofilm formation and spreading mode of microbial structures differs be...

We assessed the in vitro activity of Manuka honey against biofilm bacteria in combination with antibiotics and visualized the effect of Manuka honey on bacterial biofilms using scanning electron microscopy. The fractional biofilm eradication concentration (∑FBEC) index for vancomycin plus Manuka honey against S. aureus IDRL-4284 biofilms was 0.34, indicating a synergistic interaction. The ∑FBEC...

The aim of this study was to investigate the structure of intact oral biofilms using confocal laser scanning microscopy (CLSM). Mixed-species biofilms were grown on enamel discs in a constant depth film fermentor. The biofilms were fed with a mucin-containing artificial saliva with or without sucrose supplementation. Biofilms were examined using a Wild-Leitz CLSM, operating in reflected light m...

The paper describes an experimental study concerning the mechanical properties of bacterial biofilms formed from the early dental plaque colonizer Streptoccocus mutans and pond water biofilms. Experiments reported in this paper demonstrate that both types of biofilms exhibit mechanical behavior similar to that of rheological fluids. The time-dependent properties of both biofilms have been model...

P. aeruginosa forms biofilms in the lungs of individuals with cystic fibrosis (CF); however, there have been no effective model systems for studying biofilm formation in the CF lung. We have developed a tissue culture system for growth of P. aeruginosa biofilms on CF-derived human airway cells that promotes the formation of highly antibiotic-resistant microcolonies, which produce an extracellul...

Environmental mycobacteria are capable of forming biofilms in low-nutrient environments, and these biofilms may act as reservoirs for opportunistic infections. The purpose of this study was to determine if bacteriophages could disrupt existing biofilms of acid-fast staining Mycobacterium smegmatis. Using the MBEC 96-well plastic peg assay system, M. smegmatis biofilms were created and then test...