ESTHER TITOS,1 NAN CHIANG,2 CHARLES N. SERHAN,2 MARIO ROMANO,3 JOAN GAYA,4 GLORIA PUEYO,5 AND JOAN CLÀRIA1 1DNA Unit and 4Hormonal Laboratory, Institut d’Investigacions Biomèdiques August Pi i Sunyer, Hospital Clı́nic and 5Quı́mica Farmacéutica Bayer (Consumer Care Division), Barcelona 08036, Spain; 2Center for Experimental Therapeutics and Reperfusion Injury, Department of Anesthesiology, Periop...

Pseudomonas aeruginosa (P. aeruginosa) is a gram-negative opportunistic pathogen and a major cause of nosocomial infections. The pathogenicity of P. aeruginosa is multifactorial and associated with cell related alginates, pili and lipopolysaccharides as well as extracellular virulence factors such as proteases, haemolysins, toxins and pyocyanins. Its adaptability and high intrinsic antibiotic r...

Radioautographic and radiochemical techniques were used to establish the presence of replicating DNA in the chloroplasts of Acetabularia mediterranea. These techniques also demonstrated that these chloroplasts synthesize RNA. It was found that label from thymine was also incorporated into DNA and RNA in these chloroplasts. With the establishment of protein and nucleic acid synthesis in Acetabul...

Planaria possess a population of pluripotent adult stem cells called neoblasts, capable of differentiating into all cell types required to regenerate entire animals from almost any fragment. The regeneration of different fragments is catalogued in a body of classical experiments by many eminent scientists. Here, we endeavour to explore these classical observations and insights and combine them ...

In aquatic invertebrates, metabolic nitrogenous waste is excreted predominately as ammonia. Very little is known, however, of the underlying mechanisms of ammonia excretion, particularly in freshwater species. Our results indicate that in the non-parasitic freshwater planarian Schmidtea mediterranea, ammonia excretion depends on acidification of the apical unstirred layer of the body surface an...

Marinomonas mediterranea is a melanogenic marine bacterium that expresses two different polyphenol oxidases. One of them is a multipotent laccase able to oxidize a wide range of substrates. The second enzyme is an SDS-activated tyrosinase. Using transposon mutagenesis, a mutant affected in the regulation of both polyphenol oxidase activities and melanogenesis has been isolated. The sequencing o...

Planaria possess a population of pluripotent adult stem cells called neoblasts, capable of differentiating into all cell types required to regenerate entire animals from almost any fragment. The regeneration of different fragments is catalogued in a body of classical experiments by many eminent scientists. Here, we endeavour to explore these classical observations and insights and combine them ...