Klebsiella pneumoniaeisolates that produce carbapenemases (KPCs) are rapidly disseminating worldwide. To determine their genetic background, we investigated 16 blaKPC-2-harboring K. pneumoniae isolates from 5 countries. The isolates were multidrug resistant, possessed the blaKPC-2 gene, and differed by additional Beta-lactamase content. They harbored a naturally chromosome-encoded bla gene (bla...

Klebsiella pneumoniae is a major cause of nosocomial infections. Emergence of antibacterial resistance and production of beta-lactamases are responsible for the frequently observed empirical therapy failures. The aim of this study was to determine the presence and the prevalence of extended spectrum beta-lactamases (ESBLs) and AmpC beta-lactamases in clinical isolates of K. pneumoniae in Kerman...

1. Eckert C, Gautier V, Arlet G. DNA sequence analysis of the genetic environment of blaCTX-M genes. J Antimicrob Chemother 2006; 57: 14–23. 2. Cantón R, Coque TM. The CTX-M b-lactamase pandemic. Curr Opin Microbiol 2006; 9: 466–75. 3. Partridge SR, Hall RM. In34, a complex In5 family class 1 integron containing orf513 and dfrA10. Antimicrob Agents Chemother 2003; 47: 342–9. 4. Valverde A, Cant...

conclusions the rapid dissemination of non clonal multi-resistant ctx-m-1 producing e. coli isolates approved the need for further studies in our medical centers. results a total of 140(70%) non-duplicate esbl positive e. coli were determined. imipenem and amikacin were effective against 100% and 85% of all tested isolates respectively. co-resistance of esbl positive isolates to erythromycin, t...

Antibiotic resistance has become a global threat in which the anthropogenically influenced aquatic environment represents not only reservoir for spread of antibiotic resistant bacteria (ARB) among humans and animals but also an where genes are introduced into natural microbial ecosystems. Wastewater is one sources resistance. The aim this research was evaluation wastewater impact on water envir...

Extended-spectrum beta-lactamases (ESBL) mediating resistance to 3rd generation cephalosporins are a major public health issue. As food may be a vehicle in the spread of ESLB-producing bacteria, a study on the occurrence of cephalosporin-resistantu Escherichia coli in food was initiated. A total of 404 ESBL-producing isolates were obtained from animal-derived food samples (e.g., poultry product...

The Dilúvio stream is part of an important watershed in the city of Porto Alegre, southern Brazil. It receives rain runoffs and domestic and hospital sewage, which carries a highly diverse microbial population. Some of these microorganisms may show resistance to different antimicrobials, acting as disseminators of resistance genes. This study characterizes the Gram-negative population present i...

Studies of the transmission epidemiology of antimicrobial-resistant Escherichia coli, such as strains harboring extended-spectrum beta-lactamase (ESBL) genes, frequently use selective culture of rectal surveillance swabs to identify isolates for molecular epidemiological investigation. Typically, only single colonies are evaluated, which risks underestimating species diversity and transmission ...

OBJECTIVES The spread of ESBL-producing Enterobacteriaceae among food animals/products has raised concerns about their possible transmission through the food chain. We aimed to characterize piggeries (pigs, piggery environments) as reservoirs of TEM-52- and CTX-M-encoding plasmids and clones. METHODS Forty-three samples from five Portuguese intensive production farms were studied (2006-07). T...

Of 59 clinical isolates of Enterobacter cloacae from a teaching hospital in Sichuan, China, 18 isolates were shown to be resistant to oxyimino cephalosporins and aztreonam. Enterobacterial repetitive consensus PCR revealed that these isolates comprised 7 distinct genotypes. The presence of plasmids in the 18 clinical isolates was revealed by conjugational transfer of plasmids from E. cloacae to...