PURPOSE To identify a novel biomarker for bladder cancer targeting therapy. EXPERIMENTAL DESIGN The human bladder cancer cell line T24 cells were used as immunogen to generate mouse monoclonal antibodies. We screened and identified a specific antibody BCMab1 against bladder cancer. We examined BCMab1 antigen expression in the patients with bladder cancer through immunohistochemical staining a...

Bladder cancer (BC) is the most common urinary system malignancy worldwide. However, molecular mechanisms underlying its progression remain largely unexplored. Accumulating evidence indicates that cancer-associated fibroblasts (CAFs), major constituents of tumor stroma, play a key role in development. Herein, we have successfully isolated CAFs and paired normal (NFs) from bladder tissues. We ob...

BACKGROUND Recent meta-analyses have suggested a modest protective effect of high levels of physical activity on developing both prostate and bladder cancer, but significant heterogeneity between studies included in these meta-analyses existed. To our knowledge, few Chinese studies investigated the association between physical activity and prostate cancer and none between physical activity and ...

Killer activity of peripheral blood mononuclear cells with or without adding interleukin-2 in vitro was measured in 12 patients with superficial bladder cancer, 12 patients with invasive bladder cancer and 13 adult healthy controls. Cultured cell lines, K562, Raji and T-24 (bladder carcinoma), were served as target cells. Killer activity was measured by a 4-hour 51Cr-release assay. Mononuclear ...

UNLABELLED Long non-coding RNAs (lncRNAs) play important roles in diverse biological processes, such as transcriptional regulation, cell growth and tumorigenesis. However, little is known about whether lncRNA-GAS5 (growth arrest-specific 5) regulates bladder cancer progression. In the present study, we found that the GAS5 expression is commonly downregulated in bladder cancer cell lines and hum...

WD repeat domain 5 (WDR5) plays an important role in various biological functions through the epigenetic regulation of gene transcription; however, its role in bladder cancer remains largely unknown. Our study investigated the role of WDR5 in bladder cancer and demonstrated that WDR5 was upregulated in bladder cancer tissues, and elevated WDR5 protein levels positively correlated with advanced ...

Bladder cancer remains one of the most expensive cancers to treat in the United States due to the length of required treatment and degree of recurrence. In order to treat bladder cancer more effectively, targeted therapies are being investigated. In order to use targeted therapy in a patient, it is important to provide a genetic background of the patient. Recent advances in genome sequencing, a...

Major clinical issues in bladder cancer include the identification of prediction markers and novel therapeutic targets for invasive bladder cancer. In the current study, we describe the isolation and characterization of a tumor-initiating cell (T-IC) subpopulation in primary human bladder cancer, based on the expression of markers similar to that of normal bladder basal cells (Lineage-CD44(+)CK...

Bladder tumors represent a special therapeutic challenge as they have a high recurrence rate requiring repeated interventions and may progress to invasive or metastatic disease. Exosomes carry proteins implicated in bladder cancer progression and have been implicated in bladder cancer cell survival. Here, we characterized exosome uptake and internalization by human bladder cancer cells using Am...