نتایج جستجو برای: bovine serum

تعداد نتایج: 362357  

Journal: :Infection and immunity 1983
Y Fukazawa K Kagaya Y Ishibashi

Immune mice which exhibited a delayed-type hypersensitivity reaction to bovine serum albumin after bovine serum albumin immunization and stimulation and normal mice that had been transferred with a lymphokine-rich fraction from the supernatant of concanavalin A-stimulated spleen cell cultures demonstrated resistance to Salmonella infection.

2012
K Ojha

The dependence of nanoparticle concentration on the conformation of bovine serum albumin has been studied. A very small but consistent enhancement in the tryptophan emission is observed in the presence of sub-picomolar concentration of gold nanoparticles. At this concentration, the bovine serum albumin unfolding profile in presence and absence of nanoparticles are quite similar and the protein ...

2014
Shaida Moghaddassi Will Eyestone Colin E. Bishop

As an initial step towards creating genetically modified cattle as a biopharming source of recombinant human serum albumin (rHSA), we report modification of the bovine albumin (bA) locus by transcription activator-like effector nuclease (TALEN)-stimulated homology-directed repair (HDR). Pedigreed bovine fibroblasts were co-transfected with TALENs and an 11.5-kb human serum albumin (HSA) minigen...

2016

Submit Manuscript | http://medcraveonline.com Abbreviations: BSA: Bovine Serum Albumin; CD4: Cluster of Differentiation 4; CPS: Capsular Polysaccharide; CXCL10: Chemokine X Chemotactic Ligand 10; DHBA: Dihydroxybenzoic Acid; ER Stress: Endoplasmic Reticulum Stress; FBS: Fetal Bovine Serum; HLA: Human Leukocyte Antigen; IP-10: InterferonInducible Protein 10; LCN2: Lipocalin 2; NGAL: NeutrophilGe...

2015
Jae Eun Lee Jae Young Lee Hong Rye Kim Hyun Young Shin Tao Lin Dong Il Jin

Two dimensional-fluorescence difference gel electrophoresis (2D DIGE) is an emerging technique for comparative proteomics, which improves the reproducibility and reliability of differential protein expression analysis between samples. The purpose of this study was to investigate bovine pregnancy-specific proteins in the proteome between bovine pregnant and non-pregnant serum using DIGE techniqu...

Journal: :Chemical communications 2013
Jia Zhang Chuanxia Chen Xiaowen Xu Xiaolei Wang Xiurong Yang

We have developed a Boolean NAND logic gate for the fluorescent detection of nitrite ions by using bovine serum albumin (BSA) functionalized gold nanoclusters.

Journal: :The Biochemical journal 1970
R I Krieger C F Wilkinson

1. A potent inhibitor of microsomal mixed-function oxidation has been isolated from the gut contents of larvae of the southern armyworm (Prodenia eridania). 2. Progressive inhibition is associated with a soluble proteinase with a molecular weight of approx. 26000. 3. Although the proteolytic activity of the inhibitor can be reduced by phenylmethanesulphonyl fluoride and other reagents, these ma...

Journal: :Biophysical journal 2004
Lilia A Chtcheglova George T Shubeita Sergey K Sekatskii Giovanni Dietler

A new method of direct and continuous measurement of the spring constant of single molecule or molecular complex is elaborated. To that end the standard force spectroscopy technique with functionalized tips and samples is combined with a small dithering of the tip. The change of the dithering amplitude as a function of the pulling force is measured to extract the spring constant of the complex....

Journal: :Biophysical journal 2003
J Bloustine V Berejnov S Fraden

A method is presented for determining second virial coefficients (B(2)) of protein solutions from retention time measurements in size exclusion chromatography. We determine B(2) by analyzing the concentration dependence of the chromatographic partition coefficient. We show the ability of this method to track the evolution of B(2) from positive to negative values in lysozyme and bovine serum alb...

Journal: :American journal of clinical pathology 1986
P J Howanitz J H Howanitz C A Skrodzki T Woloszyn

Many protein methods are used for estimation of tissue receptor concentration. The authors compared performance, analytic variability, and accuracy of six protein methods used in these calculations. They found the Lowry protein procedure standardized with bovine serum albumin (BSA), usually considered the reference method, to be the most imprecise and most time consuming method. When the BSA st...

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