Herein, we report the fabrication of protein (bovine serum albumin, BSA) particles which were rendered transiently insoluble using a novel, reductively labile disulfide-based cross-linker. After being cross-linked, the protein particles retain their integrity in aqueous solution and dissolve preferentially under a reducing environment. Our data demonstrates that cleavage of the cross-linker lea...

There are some theoretical arguments related to interpreting the adiabatic compressibility (beta(s)) of a protein determined from the sound velocity and the difference between beta(s) and isothermal compressibility (beta(T)). To address these problems experimentally, we constructed a high-pressure oscillating densitometer and used it to measure the apparent specific volume of bovine serum album...

in the present study, the binding isotherms for interaction of a homologous series of n-alkyltrimethyl ammonium bromides with bovine serum albumin (bsa) have been analyzed on basis of intrinsic thermodynamic quantities. in this regards, the intrinsic gibbs free energy of binding, agb(i,)„ has been estimated at various surfactant concentrations and its trend of variation for both binding sets ha...

In this work, we present a methodology for choosing the best experimental conditions for transferring protein Langmuir films onto solid substrates. As an example of applying the proposed methodology, we used monolayers of the protein bovine serum albumin, which is a very stable protein and is of great interest in the development of immunosensors. Langmuir-Blodgett (LB) films of this protein, on...

The Standards Committee of the AACC presents a discussion of the nature of total serum protein and the problems associated with its determination. Proposals are made that protein be defined as polypeptide material, and that a reference preparation be promuJgted for interlaboratory use. The reference material suggested is bovine serum albumin, produced to rigid specifications and distributed as ...

Steady-state conductivity measurements and dielectric measurements in the frequency range 10(-5) to 100 Hz are reported for samples of bovine serum albumin, casein, and lysozyme complexed with methylglyoxal. Compared with the untreated proteins, the brown complexed proteins exhibit an increased conductivity and free electron spin density, together with a low-frequency dielectric dispersion. The...

The effect of heat on the allergenicity of beef and bovine serum albumin was investigated among 10 toddlers skin prick test (SPT)-positive to raw and cooked beef. The meat-allergy diagnosis was confirmed during double-blind, placebo-controlled food challenge (DBPCFC) with 180 g of beef cooked for 5 min at 100 degrees C. SPT with homogenized and freeze-dried beef, and heated and unheated bovine ...