The induction of apoptosis by bovine viral diarrhoea virus (BVDV) was examined in bovine peripheral blood mononuclear cells (PBMC) incubated with an antigenically homologous pair of non-cytopathic and cytopathic BVDV. Our results show that the cytopathic biotype, in contrast to the non-cytopathic counterpart, induces apoptosis in PBMC. Flow cytometry analysis of cells undergoing apoptosis revea...

The serum-neutralization (SN) and the indirect-immunofluorescence (IIF) assays have invariably been used for detecting antibodies against bovine virus diarrhoea virus (BVDV) in cattle sera. An enzyme-linked immunosorbent assay (ELISA) was applied which has a sensitivity comparable with the SN and IIF in detecting antibody to BVDV. A total of 472 bovine sera were assayed and a high prevalence of...

Bovine viral diarrhoea virus (BVDV) isolates infect cultured Madin-Darby bovine kidney (MDBK) cells as efficiently as sheep kidney cells. In contrast, border disease virus (BDV) propagates poorly in MDBK cells but infects sheep cells very efficiently. The envelope glycoprotein E2 has been shown to be essential for virus infectivity. To explore the potential role of E2 in pestivirus host range i...

Pathogens causing bovine respiratory tract disease in Finland were investigated. Eighteen cattle herds with bovine respiratory disease were included. Five diseased calves from each farm were chosen for closer examination and tracheobronchial lavage. Blood samples were taken from the calves at the time of the investigation and from 86 calves 3-4 weeks later. In addition, 6-10 blood samples from ...

Pestiviruses initiate infection of susceptible cells by receptor-mediated endocytosis. Cellular plasma membrane or endosomal molecules involved in translocation of these viruses into the cytosol have not been unequivocally identified. We reported previously that a mutant cell line derived from Madin-Darby bovine kidney (MDBK) cells, termed CRIB-1, was resistant to infection with bovine viral di...