The aim of the present study was the decolourisation of mixture of two dyes belonging to different groups by two Pseudomonas fluorescens strains (Sz6 and SDz3). Influence of different incubation conditions on decolourisation effectiveness was evaluated. Dyes used in the experiment were diazo Evans blue (EB) and triphenylmethane brilliant green (BG). Another goal of the experiment was the estima...

BACKGROUND Gene reporter-fluorescent cells have emerged as alternative method for drug screening. OBJECTIVE Achievement of constitutive expression of fluorescent protein GFP by Leishmania braziliensis as alternative method for drug screening. METHODS L. braziliensis-GFP was generated using Leishmania tarentolae pLEXSY-egfp for constitutive expression of GFP. Fluorescent cells were selected ...

Ordered array of Au semishells on TiO(2) spheres with controlled size are prepared by combining the nanosphere self-assembly and atomic layer deposition (ALD). This ordered 2-D structure with designed array of metal nanogaps can be used as an ultrasensitive surface-enhanced Raman scattering (SERS) substrate with high reproducibility and stability. More importantly, the SERS substrates are recyc...

The vacuolar surface of Nitella is covered with a non-aqueous film too thin to be visible as a separate membrane. The motion of the protoplasm may subject this film to a good deal of mechanical disturbance. Apparently this does not rupture the film for no dye escapes into the protoplasm as the result of such disturbance when the vacuolar sap is deeply stained with neutral red or brilliant cresy...

A staining procedure employing oil red O and Coomassie R-250 was developed to increase visualization of mycoplasma colonies. This procedure permits CFU determination of mycoplasmas without additional microscopy.

Before, during and after habitat restoration from 1984 to 1994, we monitored population size of the federally listed endangered El Segundo blue butterfly, Euphilotes bernardino allyni (Shields). In the subsequent formalization of a recovery plan for the species, the U.S. Fish and Wildlife Service established several recovery criteria, including a requirement of ‘a scientifically credible monito...

The terms “Pink Puffers” and “Blue Bloaters” have been around for many years. They are used in a colloquial sense by speleologists to describe how cavers react to an elevated concentration of carbon dioxide (CO2) and reduced oxygen (O2) in a cave’s “foul air” atmosphere. Foul air is only found in a small number of Australian caves and is defined as having a noticeable abnormal physiological eff...

Stroke is a leading cause of death and long-term disability. Methylene blue, a drug grandfathered by the Food and Drug Administration with a long history of safe usage in humans for treating methemoglobinemia and cyanide poisoning, has recently been shown to be neuroprotective in neurodegenerative diseases and brain injuries. The goal of this paper is to review studies on methylene blue in expe...

Guinea-pigs, rats, dogs, rabbits, monkeys, sheep, and goats were screened for G-6PD activity in erythrocytes by the methaemoglobin reduction test, the brilliant cresyl blue decolorization test, and the spectrophotometric determination of the NADPH generation rate. Guinea-pigs, rats, dogs, rabbits, and monkeys were found to have normal G-6PD activity in erythrocytes, whereas sheep and goats were...