staff Coomassie Brilliant Blau G250. J Clin Chem Cm Biochem 1981;19:203-8. 5. Peterson GL Determination of total protein.Methods Enzymol 1983;91:95-119. 6. SedmakJJ,GroesbergSE.A rapid, sensitive, and versatile assay for protein using Coomassie Brilliant Blue G250. Anal Biochem 1977;79:544-52. 7. JohnsonA, Lott JA. Standardization of the Coomassie Blue method forcerebrospinal fluid proteins. Cl...

The Piedmont enjoys a highly regarded reputation for its historic sites and its world-class preservation practices. The well-preserved homes of presidents and extraordinary examples of architecture, known worldwide, dominate our landscape. We see daily the many ways in which preservation work enriches the lives of people who live in, work in, and visit our community. Numerous residents in the P...

Expelling of dyestuff into water resource system causes major thread to the environment. Adsorption is the cost effective and potential method to remove the dyes from the effluents. Therefore, an attempt was made to study the adsorption of dyestuff (Methylene Blue (MB), Bromophenol Blue (BPB) and Coomassie Brilliant Blue (CBB)) by α-chitin nanoparticles (CNP) prepared from Penaeus monodon (Fabr...

Increasing environmental pollution is connected with broad applications of dyes and imperfection of dyeing technology. Decolourization of triphenylmethane brilliant green and disazo Evans blue by bacterial and fungal strains and toxicity (phyto- and zootoxicity) of degradation by-products were investigated. Influence of incubation method on dyes removal was evaluated (static, semi-static, shake...

AIM The aim of this study was to investigate in vitro the utility of biologically compatible, nontoxic and cell-specific targetable hydrogel nanoparticles (NPs), which have Coomassie® Brilliant Blue G dye (Sigma-Aldrich, MO, USA) covalently linked into their polyacrylamide matrix, as candidates for photothermal therapy (PTT) of cancer cells. MATERIALS & METHODS Hydrogel NPs with Coomassie Bri...

In order to obtain a compound stain to differentiate between mast and plasma cells, it was necessary to find dyes which provided a distinct colour contrast between the two types of cell. The aqueous acetic Alcian blue method (McManus and Mowry, 1960) stained mast cells, but also other tissue elements. Using Alcian blue at a pH less than 10 (Johnson and Helwig, 1963) the mast cells stained brill...

In previous studies, it was established that chitosan and its quaternized derivatives are potent enhancers of hydrophilic compounds absorption across intestinal epithelia. The aim of this study was to evaluate the application of a new quaternized chitosan, triethyl chitosan (TEC), in pharmaceutical approaches. TEC was synthesized by a one step process via a 2(2) factorial design to optimize the...