BACKGROUND Gene reporter-fluorescent cells have emerged as alternative method for drug screening. OBJECTIVE Achievement of constitutive expression of fluorescent protein GFP by Leishmania braziliensis as alternative method for drug screening. METHODS L. braziliensis-GFP was generated using Leishmania tarentolae pLEXSY-egfp for constitutive expression of GFP. Fluorescent cells were selected ...

We present an approach to intrusion detection (ID) sensor correlation that considers the problem in three phases: event aggregation, sensor coupling, and meta alert fusion. The approach is well suited to probabilistically based sensors such as EMERALD eBayes. We demonstrate the efficacy of the EMERALD alert thread mechanism, the sensor coupling in eBayes, and a prototype alert fusion capability...

While trying to develop methods for finding dehydrogenase mutants, we found tetrazolium (triphenyl tetrazolium chloride) to be useful for detecting fermentative variants. This indicator has the advantage that the nonfermenting variants are stained a deep red whereas the fermenters and the background are neutral. For example, cultures of Escherichia coli K-12 were spread on nutrient agar plates ...

β-Arrestins regulate G protein-coupled receptor signaling as competitive inhibitors and protein adaptors. Low molecular weight biased ligands that bind receptors and discriminate between the G protein dependent arm and β-arrestin, clathrin-associated arm of receptor signaling are considered therapeutically valuable as a result of this distinctive pharmacological behavior. Other than receptor ag...

The histological differentiation of neuronal from glial nuclei may be useful in the identification of tumours and in the study of developing nervous tissues. The following method does this by staining the glial nucleus red and the neuronal nucleus green. It also stains other structures in the neural parenchyma. Formol-fixed tissues taken down to water and embedded in paraffin are used. 1 The se...

A new kinetic method for the determination of Hg(II) based on its inhibitory effect on the Brilliant green-sulfite reaction at pH 6.5 is proposed. The reaction is followed spectrophotometrically at 615 nm by measuring the change in absorbance at the fixed time of 3.5 min after the initiation of the reaction. Both the influence of the reaction variables and the interference of many ions have...