Many investigators believe that immunotherapy will be the only selected treatment for leishmaniasis in the future. Leishmanization, vaccination or injection of stimulating mediators are among various forms of immunotherapy. In this pilot study, 5 volunteers with cutaneous leishmaniasis due to L.tropica were treated with 4 weekly intralesional injections of autologous buffy coat. Cytological and...

Human cytomegalovirus (HCMV) DNA quantitation in whole blood (WB) by real-time or quantitative polymerase chain reaction (qPCR) is a highly sensitive and reproducible diagnostic procedure for monitoring HCMV DNAemia (DNAemia the detection of samples plasma, blood, isolated peripheral leukocytes buffy-coat specimens) patients. We provided comparative analysis extraction performance two different...

THE finding of megaloblasts in the peripheral blood is often regarded as an uncommon event, confined to patients with severe anaemia. Methods of concenitrating the megaloblasts by making preparations from the "buffy coat'i of the haematocrit have been described (Goodall, 1957, Whitby and Britton, 1957). This paper records our experience of examining the peripheral blood for megaloblasts. METHOD...

Four patients, including three with the acquired immunodeficiency syndrome (AIDS), were treated with high-dose, buffy coat-derived alpha-interferon for progressive cytomegalovirus retinitis. Two of these patients had decreased viruria during therapy and the other two had increased viruria. There was evidence of progression of disease despite therapy in all patients, although the retinitis event...

Sir, Chanarin‐Dorfman syndrome (CDS) is a rare inherited disorder characterized by congenital ichthyosis, nonbullous ichthyosiform erythroderma, myopathy, and hepatic involvement with deposition of neutral lipids in skin, muscle, liver, central nervous system, and granulocytes. Variable skin and systemic involvement makes diagnosis difficult in some cases wherein an easy screening tool in the f...

A semicomatose 34-year-old man had fever, heart murmur, and multiple gangrenous lesions. Several blood cultures were negative. Cultures of the heart valve grew beta-haemolytic streptococcus group B. The organism was seen in buffy-coat examination of the venous blood. The gas-liquid chromatogram of the serum was different from those of normal controls and of patients with invasive candida infect...