in sturgeon aquaculture, where the main purpose is caviar production, a reliable method is needed to separate fish according to gender. currently, due to the lack of external sexual dimorphism, the fish are sexed by an invasive surgical examination of the gonads. development of a non-invasive procedure for sexing fish based on genetic markers is of special interest. in the present study we empl...

in sturgeon aquaculture, where the main purpose is caviar production, a reliable method is needed to separate fish according to gender. currently, due to the lack of external sexual dimorphism, the fish are sexed by an invasive surgical examination of the gonads. development of a non-invasive procedure for sexing fish based on genetic markers is of special interest. in the present study we empl...

Biological assay has been based on analysis of all individuals collected from sample populations. Bulked sample analysis (BSA), which works with selected and pooled individuals, has been extensively used in gene mapping through bulked segregant analysis with biparental populations, mapping by sequencing with major gene mutants and pooled genomewide association study using extreme variants. Comp...

Identification of genomic regions associated with a phenotype of interest is a fundamental step toward solving questions in biology and improving industrial research. Bulk segregant analysis (BSA) combined with high-throughput sequencing is a technique to efficiently identify these genomic regions associated with a trait of interest. However, distinguishing true from spuriously linked genomic r...

We developed bulked segregant analysis as a method for rapidly identifying markers linked to any specific gene or genomic region. Two bulked DNA samples are generated from a segregating population from a single cross. Each pool, or bulk, contains individuals that are identical for a particular trait or genomic region but arbitrary at all unlinked regions. The two bulks are therefore genetically...

Canola quality Brassica juncea has been under development at the Saskatoon Research Centre for several years. Breeding efforts have focused on the development of germplasm with improved seed quality characteristics including superior fatty acid profiles, low glucosinolates and high oil content. Future breeding efforts in this crop would be greatly facilitated through the development of molecula...

Total flower and pod numbers (TFPN) effective per plant (PNPP) are among the most important agronomic traits for soybean production. However, underlying genetic mechanism remains unclear. In this study, we constructed a recombinant inbred line population derived from cross between JY73 (high TFPN) TJSLH (low to map loci two traits. total, six QTL TFPN five PNPP were identified, which on chromos...

In this study we investigated the genetic control of avirulence in the diploid oomycete pathogen Phytophthora infestans, the causal agent of late blight on potato. The dominant avirulence (Avr) genes matched six race-specific resistance genes introgressed in potato from a wild Solanum species. AFLP markers linked to Avr genes were selected by bulked segregant analysis and used to construct two ...

Rust, incited by Uromyces appendiculatus^ is a major disease in common bean {Phaseolus vulgaris L,). Plant growth habit is also an important trait. Indeterminate growth habit was first reported to be controlled by a single dominant gene (Fin) (Lamprecht, 1935). Molecular markers such as isozymes, RFLP, and RAPD, have been used to tag genes for many traits, particularly disease resistance in sev...