An efficient procedure has been developed for callus induction and cell suspension cultures of C. saxicola for the first time. Explant selection was carried out among leaf, stem and root to select a suitable type of explants capable of higher callus formation. Leaf explants thus selected showed maximum response to callus induction (67.1%). Modified B5 medium supplemented with 0.5 mg l(-1) 2,4-D...

Objective: n present study effect of various factors including sucrose, agar, PEG, AgNO3, activated charcoal on somatic embryo induction and its subsequent regeneration was investigated using varieties Dom siah and Nemat. Methods: 330 explant (Mature embryos) of each varieties were cultured, 33/plate (100 x 15 mm), on callus induction Murashige and Skoog (MS) medium (Murashige and Sk...

In this review, we report on the different plant growth regulators (PGRs) affecting callus induction, callus-mediated regeneration, organogenesis, somatic embryogenesis from explants and micropropagation through shoot tip nodal cultures in Turkey Berry (Solanum torvumSW). The fruits of guard against liver kidney damage, stop certain cancers, regulate blood sugar levels. They benefit digestion, ...

Objective: To develop in vitro callus induction in nodal segments of Decalepis hamiltonii and investigate the antioxidant potential of the callus. Methods: In this study, nodal segments of Decalepis were cultured on Murashige and Skoog (MS) medium supplimented with various concentration of 2,4-D for callus induction and later subcultured on various concentration of NAA and BAP. The antioxidant ...

The antimicrobial activity of plant extracts were found in folk medicine as essential oils or isolated compounds. Some of these compounds were isolated or obtained by bio-guided isolation after previously detecting antimicrobial activity on the part of the plant. Petunia is an imporant medicinal and ornamental plant.The in vitro callus induction and antibacterial activity of Petunia leaf and ca...

The medicinal plant Plumbago contains a very potent secondary metabolite, plumbagin having many therapeutic properties. Callus culture was induced using explants, leaf, stem and shoot apex, from P. auriculata. Murashige and Skoog media fortified with various growth hormones like NAA, IAA, IBA and 2, 4-D individually and in various combinations were checked for callus induction. Among the growth...

Leaf and stem explants excised from young plant of B.diffusa  were cultured on Murashige and Skoog (MS) medium containing agar (0.8%), sucrose (2.5%) and varied concentration of Indole butyric acid (IBA), Napthyl acetic acid (NAA), 2,4 - dichloro phenoxy acetic acid (2,4-D) and Picrolam. Leaf explants has given better response for both rooting and callus formation. IBA in a concentration of 5μM...

Callus cultures from root explants of Lathyrus sativus L. ‘Derek’ were tested for their morphogenic capacity. Primary explants (fragments of roots) were cultivated on three induction media. We obtained three lines of callus tissue among which we identified two non-embryogenic lines and one embryogenic line. Callus originally cultivated on modified MS medium supplemented with 0.05 mgL picloram, ...