Activation of caspase-1 through the inflammasome protein Nalp1b controls anthrax lethal toxin (LT)-induced necrosis in murine macrophages. In this study we analyzed physiological changes controlled by caspase-1 in LT-treated murine macrophages. The caspase-1 inhibitor Boc-D-cmk blocked caspase-1 activity and membrane impairment in LT-treated cells. To determine the relationship between caspase-...

BACKGROUND The significance of caspase-1 in prostate cancer has recently been documented (Cancer Res 61: 1227-1232, 2001). In this study, we investigated the role of caspase-1 in radiation-induced apoptosis in order to identify the significance of this apoptotic initiator in radiation resistance. MATERIALS AND METHODS Caspase-1 was over-expressed in DU-145 prostate cancer cells (which have we...

Necroptosis is a regulated necrotic-like cell death modality which has come into the focus of attention since it known to contribute pathogenesis many inflammatory and degenerative diseases as well tumor regulation. Based on current data, necroptosis serves backup mechanism when receptor-induced apoptosis inhibited or absent. However, necroptotic role proteins involved in mitochondrial not been...

RATIONALE Endothelial injury is an initial mechanism mediating cardiovascular disease. OBJECTIVE Here, we investigated the effect of hyperhomocysteinemia on programed cell death in endothelial cells (EC). METHODS AND RESULTS We established a novel flow-cytometric gating method to define pyrotosis (Annexin V(-)/Propidium iodide(+)). In cultured human EC, we found that: (1) homocysteine and l...

Objectives Pyroptosis, a special kind of cell death, is generally believed to aggravate the inflammatory response. It has been found that acinar pyroptosis exits in course pancreatitis. Ulinastatin function inhibiting release mediators, thereby reducing reaction, which suggests ulinastatin potential inhibitory effect on pyroptosis. The aim this study investigate caspase-1-dependent acute Method...

Microcystin-LR (MC-LR) and glyphosate (GLY) have been classified as a Group 2B 2A carcinogens for humans, respectively, frequently found in aquatic ecosystems. However, data on the potential hazard of MC-LR GLY exposure to fish gut are relatively scarce. In current study, subacute toxicity test zebrafish exposed (35 μg L−1) (3.5 mg L−1), either alone or combination was performed 21 d. The resul...

Autocatalytic activation of an initiator caspase triggers the onset of apoptosis. In dying cells, caspase-9 activation is mediated by a multimeric adaptor complex known as the Apaf-1 apoptosome. The molecular mechanism by which caspase-9 is activated by the Apaf-1 apoptosome remains largely unknown. Here we demonstrate that the previously reported 1:1 interaction between Apaf-1 caspase recruitm...

cladribine acts as an antineoplastic, immunosuppressive and anti-inflammatory agent. the aim of this study was to examine the possible impact of this drug on the epidermis of wistar rats. the control group received standard feed and water. the experimental group i was treated with cladribine in a schema corresponding to the treatment of multiple sclerosis in humans. the material for microscopic...

econazole nitrate (en), a synthetic compound, is now in use as a routine antifungal drug. en was shown to have antitumor effect, the tumor cell killing mechanisms, however, remain unclear. in this research, the apoptosis-inducing effect of en on mcf-7 cells was investigated. the results showed that en inhibited the proliferation of mcf-7 cells in a time- and dose-dependent manner by mtt method ...