UV radiation from the sun activates both the membrane death receptor and the intrinsic or mitochondrial apoptotic signaling pathways in epidermal keratinocytes, triggering apoptosis and affording protection against skin cancer formation. We have investigated the involvement of caspase-9 in the UV death effector pathway in human keratinocytes, since this is the initiating caspase in the mitochon...

Nowak, Grażyna, Peter M. Price, and Rick G. Schnellmann. Lack of a functional p21WAF1/CIP1 gene accelerates caspase-independent apoptosis induced by cisplatin in renal cells. Am J Physiol Renal Physiol 285: F440–F450, 2003. First published May 13, 2003; 10.1152/ajprenal.00233.2002.— The lack of cyclin-dependent kinase inhibitor p21WAF1/CIP1 (p21) in mice increases renal proximal tubular cell de...

It was found by Motokawa1) that the indirect induction caused by a colored light at any retinal area disappears when a remote region of the retina is illuminated by another colored light complementary to the former. This phenomenon was attributed by Motokawa to a kind of intereaction between the indirect induction caused by the first colored light and a cer tain propagated process called " spre...

Epidemiological studies have evidenced that natural dietary products can prevent or manage gastric cancer. Neptunia oleracea, an aquatic vegetable and edible plant, has been reported to anti-cancer properties. In this study, N. oleracea extract’s suppression of cancer cells was investigated on in vitro experimental model. We found ethyl acetate (EtOAc) extract inhibited cell proliferation at IC...

To investigate the effect of emodin combined with Cp-1 on proliferation and apoptosis bladder cancer cell line BIU-87. BIU-87 cells were randomly divided into control group, group. Flow cytometry was used to detect cells. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay Western blot reverse transcription–polymerase chain reaction recepteur d’origine nantais, Caspase-3 B-cell ...

Delayed neuronal death after transient cerebral ischemia may be mediated, in part, by the induction of apoptosis-regulatory gene products. Caspase-3 is a newly characterized mammalian cysteine protease that promotes cell death during brain development, in neuronal cultures, and in other cell types under many different conditions. To determine whether caspase-3 serves to regulate neuronal death ...

Two novel synthetic tetrapeptides, VEID-CHO and DMQD-CHO, could selectively inhibit caspase-6 and caspase-3, respectively. We used these inhibitors to dissect the pathway of caspase activation in Fas-stimulated Jurkat cells and identify the roles of each active caspase in apoptotic processes. Affinity labeling techniques revealed a branched protease cascade in which caspase-8 activates caspase-...

The use of plasmon coupling in metal nanoparticles has shown great potential for the optical characterization of many biological processes. Recently, we have demonstrated the use of "plasmon rulers" to observe conformational changes of single biomolecules in vitro. Plasmon rulers provide robust signals without photobleaching or blinking. Here, we show the first application of plasmon rulers to ...