Free radicals play an important role in pathological processes such as aging and developing cancer; hence, natural products inhibit free radical production, and can play an important role in preventing diseases. We aimed to evaluate the scavenging activity of free radicals, reducing power, inhibition of lipid peroxidation and malondialdehyde (MDA) formation, and the antioxidant composition of r...

The enzyme 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase (EC 4.1.2.15) catalyzes the first committed step in the shikimate pathway of tryptophan synthesis, an important precursor for the production of terpenoid indole alkaloids (TIAs). A full-length cDNA encoding nuclear coded chloroplast-specific DAHP synthase transcript was isolated from a Catharanthus roseus cDNA library. This ...

BACKGROUND AND AIMS Catharanthus roseus is a highly valuable medicinal plant producing several terpenoid indole alkaloids (TIAs) with pharmaceutical applications, including the anticancer agents vinblastine and vincristine. Due to the interest in its TIAs, C. roseus is one of the most extensively studied medicinal plants and has become a model species for the study of plant secondary metabolism...

Two direct HPLC analytical methods for the screening of the major indole alkaloids of Catharanthus roseus hairy roots and their iridoid precursors have been developed. Photodiode array and fluorescence detection were performed. The separation was achieved on a reversed-phase C18 column. The first method allowed the separation of catharanthine, serpentine, tabersonine, vindoline, vinblastine, an...

High intake of dietary fructose has been shown to exert a number of adverse metabolic effects in humans and experimental animals. The aim of the present study was to investigate whether Catharanthus roseus (L.) G. Don (Apocynaceae) leaf powder alleviates high-fructose diet-induced insulin resistance and oxidative stress in rats. Adult male Wistar rats of body weight around 200 g were divided in...

Shoot biomass production, alkaloid content and composition as influence by cadmium, manganese, nickel and lead at uniform dose of 5 mM were investigated in Catharanthus roseus plants grown in sand culture. Treatment with Mn, Ni, and Pb significantly enhanced total root alkaloid accumulation. Cd and Ni treatment resulted in two-fold where as Pb treatment resulted in three fold increase in serpen...

Background & Aim: Investigation of suspension cell culture and callus production in Catharanthus roseus. Catharanthus roseus is an herbaceous perennial plant belongs to the family Apocynaceae. C. roseus is important as commercial medicinal plant because of its alkaloidal content. Whole plant contains about 130 alkaloids those are classified as vinca alkaloids...

Phytochemical screening and gas chromatography-mass spectrometry (GC-MS) methods were used to determine the phytochemical components of various extracts stems roots Catharanthus roseus. Preliminary n-hexane, chloroform, ethyl acetate methanol roseus carried out identify class compounds present in each extract. The results exhibited that steroids, terpenoids, flavonoids phenolic found every extr...