The encapsulation of cells together with micro-objects in monodispersed water-in-oil microdroplets offers a powerful means to perform quantitative biological studies within large cell populations. In such applications, accurate object detection is crucial ensure control over the content for every compartment. particular, ability rapidly count and localize objects key future applications single-...

BACKGROUND Semiquantitative evaluation and manual cell counting are the commonly used procedures to assess positive staining of molecular markers in tissue sections. Manual counting is also a laborious task in which consistent objectivity is difficult to achieve. Recently, image analysis has been explored, but the studies reported were limited to histological images acquired at high magnificati...

Counting of red blood cells (rbc) in blood cell images is very important to detect as well as to follow the process of treatment of many diseases like anaemia, leukaemia etc. However, locating, identifying and counting of -red blood cells manually are tedious and time-consuming that could be simplified by means of automatic analysis, in which segmentation is a crucial step. In this paper, we pr...

Background: Multiple myeloma (MM) is a malignant plasma cell proliferative disorder with limited immunotherapy treatment because of T cell dysfunction. Objective: To investigate the immunomodulatory function of bone marrow mesenchymal stromal cells (MM-BMSCs) on CD8+ T cells. Methods: Proliferation and cytotoxicity were detected by c...

The Vickers J12 cell counter uses an optical hydraulic counting system to perform rapid automatic counts of leucocytes and erythrocytes in liquid suspension. The effect of sample storage, the use of different diluents, variation in cell dimensions and other relevant factors have been studied. A counting accuracy of +/- 1.19% for replicate erythrocyte counts and +/- 2.94% for leucocyte counts wa...

introduction: counting is of the most basic mathematical abilities. many researches have demonstrated that different perceptual abilities can affect counting skills. we investigated the impact of visual search (vs) and visuoumotor organization (vmo) on counting speed. material  and  methods:   a  total  of  40  people  in  two  groups  of borderline  and  normal intellectual ability (20 subject...