Abstract Nucleic acid aptamers are generated by an in vitro molecular evolution method known as systematic of ligands exponential enrichment (SELEX). Various candidates limited actual sequencing data from experiment. Here we developed RaptGen, which is a variational autoencoder for silico aptamer generation. RaptGen exploits profile hidden Markov model decoder to represent motif sequences effec...

SELEX took data in the 1996/7 Fixed Target Run at Fermilab. The excellent performance parameters of the SELEX RICH Detector had direct influence on the quality of the obtained physics results.

Aptamers are high-affinity ligands selected from DNA or RNA libraries via SELEX, a repetitive in vitro process of sequential selection and amplification steps. RNA SELEX is more complicated than DNA SELEX because of the additional transcription and reverse transcription steps. Here, we report a new selection scheme, RAPID-SELEX (RNA Aptamer Isolation via Dual-cycles SELEX), that simplifies this...

Aptamers are nucleic acid molecules that have been selected in vitro to bind to their molecular targets with high affinity and specificity. Typically, the systematic evolution of ligands by exponential enrichment (SELEX) process is used for the isolation of specific, high-affinity aptamers. SELEX, however, is an iterative process requiring multiple rounds of selection and amplification that dem...

Screening for hits against a target may be performed with various screening methods, like phage display peptides and antibodies or SELEX (Systematic Evolution of Ligands by EXponential enrichment) aptamers (Figure 1). Aptamers are small oligonucleotides (typically 20–100 bases) 3D structures that bind to protein targets. used as diagnostic therapeutic tools, example perform targeted drug delive...

The selectins are calcium-dependent C-type lectins that recognize complex anionic carbohydrate ligands, initiating many cell-cell interactions in the vascular system. Selectin blockade shows therapeutic promise in a variety of inflammatory and postischemic pathologies. However, the available oligosaccharide ligand mimetics have low affinities and show cross-reaction among the three selectins, p...

Aptamers are small non-coding RNAs capable of recognizing, with high specificity and affinity, a wide variety of molecules in a manner that resembles antibodies. This class of nucleic acids is the resulting product of applying a well-established screening method known as SELEX. First developed in 1990, the SELEX process has become a powerful tool to select structured oligonucleotides for the re...

To the Editor: Revolutionizing the traditional Systematic Evolution of Ligands by EXponential Enrichment (SELEX), high-throughput SELEX (HT-SELEX) has become the method of choice for the identification of aptamers—synthetic, single-stranded (ribo)nucleic molecules that bind to a given molecular target. 1 In contrast to traditional SELEX, in which only data from the last selection cycle is obtai...