background: electroporation is a valuable tool for small interfering rna (sirna) delivery into cells because it efficiently transforms a wide variety of cell types. since electroporation condition for each cell type must be determined experimentally, this study presents an optimal electroporation strategy to reproducibly and efficiently transfect mda-mb 468 human breast cancer cell with sirna. ...

objectives: this study investigated the possible synergistic effect of simultaneous treatment of bone morphogenic protein (bmp)-4 as a chemical stimulator and static magnetic field (smf) as a physical stimulator on viability percent and proliferation rate in rat bone marrow stem cells. methods: passage 5 cells were trypsinized, and a cell suspension prepared after which the cells were counted ...

Background: Photodynamic therapy (PDT) is a promising therapeutic modality for the treatment of cancer and other diseases. In this study, the epidermoid carcinoma cell line A431 and the normal fibroblasts were used to investigate whether gold nanoparticles (GNPs) can induce an increase in cell death during PDT using 5-aminolevulinic acid (5-ALA) as a photosensitizer.Methods: Human fibroblast an...

Objective(s): Hyperglycemia, oxidative stress and apoptosis have key roles in pathogenesis of diabetic neuropathy. There are local renin-angiotensin systems (RASs) in different tissues such as neural tissue. Local RASs are involved in physiological and pathophysiological processes such as inflammation, proliferation and apoptosis. This study aimed to investigate the role of local renin-angioten...

Background: Many physical and mechanical phenomena occur during the acupuncture and tuina regime, and pressure is one of the most basic mechanical phenomena. Objectives: To understand the cellular bio-physical mechanism of basic mechanical stimulation via acupuncture and tuina by investigating the effect of different in vitro pressures on the cel...

i ntroduction: assessment of cellular cytotoxicity is a regular method for evaluating the biocompatibility of novel materials. in a recent study, 5% fluoride varnish (duraflur) has shown reasonable sealing ability and coverage of root canal walls when used as a sealer. the aim of the present study was to compare cytotoxicity of duraflur varnish with two popular commonly used root canal sealers ...

Background: Evaluation of cell viability is momentous in pharmacologic and oncological research. Cell viability evaluation determines cell sensitivity and consequently treatment outcome. Various methods are available to determine cell survival. Each of these methods evaluates different endpoints. Accordingly, determining the correlation between these methods is important. In this study, in orde...

Introduction: Based on human studies, inequality and social injustice have adverse effects on the individual and community health. In this study, the effects of food intake inequality and social status changes on pain perception and immunological factors were investigated in Balb/C mice. Methods: The present study was conducted by implementing different social stresses including food depriva...