Cellulose-derived glucose generated using the supported ruthenium catalyst was applied to poly(3-hydroxybutyrate) [P(3HB)] production in recombinant Escherichia coli. By the reaction with the catalyst at 220°C, 15-20 carbon mol% of cellulose was converted into glucose. The hydrolysate also contained byproducts such as fructose, mannose, levoglucosan, oligomeric cellulose, 5-hydroxymethylfurfura...

This study reports an ethanol yield of 270L/ton wood from lodgepole pine pretreated with sulfite pretreatment to overcome recalcitrance of lignocellulose (SPORL) using an adapted strain, Saccharomyces cerevisiae Y5, without detoxification. The enzymatic hydrolysate produced from pretreated cellulosic solids substrate was combined with pretreatment hydrolysate before fermentation. Detoxification...

Cellulosic ethanol production from lignocellulosic biomass offers a sustainable solution for transition from fossil based fuels to renewable alternatives. However, a few long-standing technical challenges remain to be addressed in the development of an economically viable fermentation process from lignocellulose. Such challenges include the needs to improve yeast tolerance to toxic inhibitory c...

The sustainable production of biofuels will require the efficient utilization of lignocellulosic biomass. A key barrier involves the creation of growth-inhibitory compounds by chemical pretreatment steps, which ultimately reduce the efficiency of fermentative microbial biocatalysts. The primary toxins include organic acids, furan derivatives, and phenolic compounds. Weak acids enter the cell an...

Background: Pretreatment chemistry is of central importance due to its impacts on cellulosic biomass processing and biofuels conversion. Ammonia fiber expansion (AFEX) and dilute acid are two promising pretreatments using alkaline and acidic pH that have distinctive differences in pretreatment chemistries. Results: Comparative evaluation on these two pretreatments reveal that (i) AFEX-pretreate...

BACKGROUND Pretreatment chemistry is of central importance due to its impacts on cellulosic biomass processing and biofuels conversion. Ammonia fiber expansion (AFEX) and dilute acid are two promising pretreatments using alkaline and acidic pH that have distinctive differences in pretreatment chemistries. RESULTS Comparative evaluation on these two pretreatments reveal that (i) AFEX-pretreate...

In the current investigation, woody stem of Moringa oleifera was processed by chipping and milling subsequently exposed to a combination pretreatments involving 3% v/v solution nitric acid autohydrolysis. The simultaneous saccharification fermentation (SSF) pretreated hydrolysate M. conducted using Zymomonas mobilis Kluyveromyces marxianus in occurrence commercial cellulase enzyme, Tween 80, so...

In order to exploit a fast-growing Paulownia hardwood as an energy crop, xylose-enriched hydrolysate was obtained in this work increase the ethanol concentration using hemicellulosic fraction, besides already widely studied cellulosic fraction. For that, elongata x fortunei submitted autohydrolysis treatment (210 °C or S0 of 4.08) for xylan solubilization, mainly xylooligosaccharides. Afterward...