Plant tissues contain abundant polysaccharides, phenolic compounds and other metabolites, which makes it difficult to isolate high-quality RNA from them. In addition, Neolamarckia cadamba contains large quantities of other components, particularly RNA-binding alkaloids, which makes the isolation even more challenging. Here, we describe a concise and efficient RNA isolation method that combines ...

Genes encoding the cellobiohydrolase enzyme (CBHI), designated as cbhI, were isolated from the basidiomycetes Auricularia fuscosuccinea, Pleurotus giganteus, P. eryngii, P. ostreatus, and P. sajor-caju. Initially, the fungal genomic DNA was extracted using a modified cetyltrimethyl ammonium bromide (CTAB) protocol and used as a DNA template. The cbhI genes were then amplified and cloned using t...

Five different RNA extraction methods have been tried out on the red seaweed, Gracilaria changii collected from the mangrove area at Morib, Selangor, Malaysia. Two methods, one utilising guanidinium thiocyanate, and another using cetyltrimethyl ammonium bromide (CTAB), were found to be potential alternatives to obtain pure RNA. By incorporating sand while grinding the tissue, the method using C...

Abstract Tantalum pentoxide nanoparticles (Ta 2 O 5 NPs) were fabricated by cathode glow discharge electrolysis (CGDE) generated between a needle-like platinum wire and tantalum foil anode in 6 g L −1 Na SO 4 electrolyte solution containing ml hydrofluoric acid (HF) 0.075 cetyltrimethyl ammonium bromide (CTAB). The chemical structure, composition morphology of the obtained powder analyzed using...

Ion pair dispersive liquid- liquid microextraction (IP-DLLME) method combined with flame atomic absorption spectrometry was proposed for the determination of trace amounts of copper(II). By using pyrocatechol violet as chelating agent and cetyltrimethyl ammonium bromide as an ion pairing agent, the trace amount of copper(II) was extracted in chloroform. The factors influencing the formation cop...

Mesoporous SiO2 nanopowder was synthesized under an acidic condition by a sol-gel method using various amounts of cetyltrimethyl ammonium bromide (CTAB) as structure directing agent. The samples were investigated with XRD, SEM, FTIR, TEM and N2 absorption-desorption analysis. Also, the incremental effect of surfactant were examined. The results obtained from the analysis suggested that an incre...

This work describes a new, low-cost and simple-to-use method for the determination of free biothiols in biological fluids. The developed utilizes interaction with gold ions, previously anchored on micellar assemblies through electrostatic interactions hydrophilic headgroup cationic surfactant micelles. Specifically, reaction AuCl4− cetyltrimethyl ammonium bromide (CTAB) produces an intense oran...