The authors demonstrate herein a novel time-resolved fluoroimmunoassay (TRFIA) protocol for quantification of human IgG with the new bifunctional chelate Eu(TTA)₃(5-NH₂-phen) (ETNP) labeling the goat anti-human IgG. The immunoassay was conducted by following the typical procedure for sandwich-type immunoreactions. Goat anti-human IgG was immobilized on aldehyde-modified glass slides. The human ...

One factor that is critical to the potential effectiveness of radioimmunotherapy is the design of radiometal-chelated antibodies that will be stable in vivo. Stability in vivo depends on the condition that both the chelate linkage and radiolabeling procedures not alter antibody specificity and biodistribution. In addition, synthesis and selection of the chelating agent is critical for each radi...

in this investigation, attempts were directed to study the inhibitive effects of nitrite and phosphate base inhibitors for the carbon steel alloy samples, which are used in an acid gas treatment process (the sulfiran® solution), in fe-edta solution. electrochemical techniques, i.e. polarization curves and electrochemical impedance spectroscopy (eis), were used to evaluate the inhibition efficie...

In order to investigate the effect of humic acid (HA) and nano-calcium chelate on the cut flowers of gerbera cv. Dune, an experiment was performed as a completely randomized design with two factors and three replications in hydroponic conditions. The first factor included humic acid at 4 concentrations of 0 (control), 500, 1000, and 2000 mg L-1 as drench and the second factor was ass...

We have developed a rapid and reliable bacteriophage quantification method based on measurement of phage single-stranded DNA (ssDNA) using switchable lanthanide chelate complementation probes. One oligonucleotide probe contains a non-fluorescent lanthanide ion carrier chelate and another probe is labeled with a light absorbing antenna ligand. Hybridization of the non-fluorescent complementation...

The carbene-C ,N chelate tetracarbonyl tungsten complex [(CO)4/ W C(Ph)NHC5H4N /-o ] (4), obtained from [(CO)5W / C(Ph)OMe] (3) and o -aminopyridine, reacts with iodine by oxidative decarbonylation to give a single isomer of the carbene-C ,N chelate tricarbonyl dihalogeno tungsten(II) complex [(CO)3I2/ W C(Ph)NHC5H4N /-o ] (5). The analogous reaction of 4 with bromine affords two interconvertin...

The antitumor activity of the copper(H) chelate of 3-ethoxy-2oxobutyraldehyde bis(thiosemicarbazone), Cu(II)KTS, against the Walker 256 carcinosarcoma in rats is shown, and the im portance of considering this chelate as the active intermediate in the mechanism of action of the free ligand, KTS, is indicated. The potentiation of the antitumor activity and toxicity of Cu(II)KTS by KTS and Vu(II) ...

holy basil (ocimum sanctum) is one of the important medicinal plant belongs to lamiaceae family. in order to evaluate the effect of foliar application of iron on iron and chlorophyll contents and photosynthetic parameters of holy basil, an experiment was conducted at research farm of department of horticultural science, shahid chamran university of ahvaz based on randomized complete block desig...

Isoxazole ring formation was examined as a potential Cu-free alternative click reaction to Cu(I)-catalyzed alkyne/azide cycloaddition. The isoxazole reaction was explored at macroscopic and radiotracer concentrations with the fac-[M(I)(CO)3](+) (M = Re, (99m)Tc) core for use as a noncoordinating linker strategy between covalently linked molecules. Two click assembly methods (click, then chelate...

To meet the continual demands of more-sensitive immunoassays, the synthesis of novel luminescent Eu(III) chelate labels having similar substituted 4-(phenylethynyl)pyridine chromophores in three different chelate structure classes are reported. Significantly enhanced luminescence intensities were obtained, evidently caused by the intra-ligand charge transfer (ILCT) mediated sensitization, but t...