Atomic force microscopy reveals that Pseudomonas aeruginosa LecA (PA-IL) and a tetra-galactosylated 1,3-alternate calix[4]arene-based glycocluster self-assemble according to an aggregative chelate binding mode to create monodimensional filaments. Lectin oligomers are identified along the filaments and defects in chelate binding generate branches and bifurcations. A molecular model with alternat...

Tridentate chelate ligands of 2,6-bis[hydroxy(methyl)amino]-1,3,5-triazine family rapidly release iron from human recombinant ferritin in the presence of oxygen. The reaction is inhibited by superoxide dismutase, catalase, mannitol and urea. Suggested reaction mechanism involves reduction of the ferritin iron core by superoxide anion, diffusion of iron(II) cations outside the ferritin shell, an...

The movement proteins of two tobamoviruses (tobacco mosaic virus, TMV, common strain U1 and cruciferous TMV strain) containing amino-terminal hexahistidine affinity tags were overexpressed in Escherichia coli and purified by metal chelate affinity chromatography. Purified recombinant proteins were immobilized to a Ni(2+)-chelate adsorbent and their ability to interact with full-length genomic T...

The Click-to-Chelate approach is a highly efficient strategy for the radiolabeling of molecules of medicinal interest with technetium and rhenium-tricarbonyl cores. Reaction of azide-functionalized molecules with alkyne prochelators by the Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC; click reaction) enables the simultaneous synthesis and conjugation of tridentate chelating systems for the...

A variety of mechanisms have been proposed for detecting inorganic ions using magnetic resonance imaging (MRI) contrast agents. In the most widely explored approach, analyte binding to a paramagnetic metal chelate withdraws ligand(s), increasing the number (q) of inner sphere sites available for water coordination at the paramagnetic center. As a consequence, the longitudinal relaxivity (r1) of...

We report a sensitive assay method for homogeneous thrombin detection. The method is based on lanthanide chelate complementation, where the luminescent complex is split into two separate label moieties, which are intrinsically non-luminescent. A luminescent mixed chelate complex is formed only when the label moieties are brought into close proximity directed by two separate binding events of ap...

The title compound, [Ir(C(11)H(8)N)(2)(C(17)H(19)O(2))], has an octa-hedral coordination geometry around the Ir(III) atom, retaining the cis-C,C,trans-N,N chelate disposition of the two 2-phenyl-pyridine ligands. The chelate rings are nearly mutually perpendicular [the inter-planar angles range from 85.48 (17) to 89.17 (19)°]. The two 2-(2-pyrid-yl)phenyl ligands are approximately planar, with ...