The so far largely uncharacterized central carbon metabolism of the yeast Pichia stipitis was explored in batch and glucose-limited chemostat cultures using metabolic-flux ratio analysis by nuclear magnetic resonance. The concomitantly characterized network of active metabolic pathways was compared to those identified in Saccharomyces cerevisiae, which led to the following conclusions. (i) Ther...

Serum free fermentation procedures of cell cultures have got a wide application in production of biochemicals. But, cells cultured in serum free media in general are more sensitive to changes in culture condition, especially to nutrient limitation. There are no substances from serum which can support the cells when conditions are changing. In this study special attention is directed to amino ac...

Protein expression is shaped by evolutionary processes that tune microbial fitness. The limited biosynthetic capacity of a cell constrains protein expression and forces the cell to carefully manage its protein economy. In a chemostat, the physiology of the cell feeds back on the growth conditions, hindering intuitive understanding of how changes in protein concentration affect fitness. Here, we...

A discrete, size-structured model of phytoplankton growth in the chemostat. A discrete, size-structured model of phytoplankton growth in the chemostat. Abstract: We introduce inhomogeneous, substrate dependent cell division in a nonlinear matrix model of size-structured population growth in the chemostat, rst introduced by Gage et al. 7] and later analyzed by Smith 11]. We show that mass conser...

The influence of global change on Southern Ocean productivity will have major ramifications for future management polar life. A prior laboratory study investigated the response a batch-cultured subantarctic diatom to projected simulating conditions 2100 (increased temperature/CO2/irradiance/iron; decreased macronutrients), showed twofold higher chlorophyll-derived growth rate driven mainly by t...

We consider the modeling of the dynamics of the chemostat at its very source. The chemostat is classically represented as a system of ordinary differential equations. Our goal is to establish a stochastic model that is valid at the scale immediately preceding the one corresponding to the deterministic model. At a microscopic scale we present a pure jump stochastic model that gives rise, at the ...

We consider the modeling of the dynamics of the chemostat at its very source. The chemostat is classically represented as a system of ordinary differential equations. Our goal is to establish a stochastic model that is valid at the scale immediately preceding the one corresponding to the deterministic model. At a microscopic scale we present a pure jump stochastic model that gives rise, at the ...

Two novel modifications of continuous culture with gradual change of dilution rate (A-stat): D-stat and auxo-accelerostat were evaluated in the studies of the effect of changing individual environmental parameters (T, pH, pO(2), substrate concentration, etc.) on growth characteristics of different microorganisms. Common for those cultivation methods is that one environmental parameter is progra...

The Rosenzweig-MacArthur (1963) criterion is a graphical criterion that has been widely used for elucidating the local stability properties of the Gause (1934) type predator-prey systems. It has not been stated whether a similar criterion holds for models with explicit resource dynamics (Kooi et al. (1998)), like the chemostat model. In this paper we use the implicit function theorem and implic...