Chromatin-immunoprecipitation (ChIP) employs generally a mild formaldehyde cross-linking step, which is followed by isolation of specific protein-DNA complexes and subsequent PCR testing, to analyze DNA-protein interactions. Poly(ADP-ribosyl)ation, a posttranslational modification involved in diverse cellular functions like repair, replication, transcription, and cell death regulation, is most ...

objectives: human immunodeficiency virus type 1 is the causative agent of acquired immunodeficiency syndrome “aids” in human and demonstration of hiv-1 genome in samples is accepted as evidence of infection. transmission of infection during window period in blood transfusion settings is a world wide concern. also there is a need for a rapid, sensitive and accurate technique to detect hiv-1 infe...

The maturation of mouse macrophages and dendritic cells involves the transient deposition of ubiquitylated proteins in the form of dendritic cell aggresome-like induced structures (DALIS). Transient DALIS formation was used here as a paradigm to study how mammalian cells influence the formation and disassembly of protein aggregates through alterations of their proteostasis machinery. Co-chapero...

Wireless network on chip (WiNoC) is one of the promising on-chip interconnection networks for on-chip system architectures. In addition to wired links, these architectures also use wireless links. Using these wireless links makes packets reach destination nodes faster and with less power consumption. These wireless links are provided by wireless interfaces in wireless routers. The WiNoC archite...

Constant quality improvement through automation of production processes is an important prerequisite for increased viability mining machines and plants. Factors that limit the cutting machining operations include problem controlling chip formation crushing. Solution this necessitates theoretical description material conditions tools with curvilinear surfaces. The paper describes basic principle...

In this paper, we describe a fabrication method of a glass microfluidic chip for long-term and repeatable lipid bilayer formation. We etched a glass substrate by magnetic neutral loop discharge plasma (NLD) (Fig. 1a). Using this method, we fabricated a glass microfluidic chip with microchambers forming lipid bilayer membranes (Fig.1b,c). Since the glass chip prevents absorption of fluids, the f...