Chitosan with a molecular weight (MW) of 10 Da and 13% degree of acetylation (DA) was extracted from the mycelia of the fungus Gongronella butleri USDB 0201 grown in solid substrate fermentation and used to prepare scaffolds by the freezedrying method. The mechanical and biological properties of the fungal chitosan scaffolds were evaluated and compared with those of scaffolds prepared using chi...

Chitosan was recently indicated to enhance osteogenesis, improve wound healing but to activate the coagulation and the complement systems. In the present study approximately 10 nm thick chitosan film were prepared on aminopropyltriethoxysilane (APTES) coated silicon. The surfaces were incubated in serum or plasma and subsequently in antibodies towards key complement and contact activation of co...

Chitosan is a biopolymer derived from chitin which is naturally occurring in the exoskeleton of crustaceans. This paper reports dissolution and regeneration of chitosan by directly dissolving in an ionic liquid solvent, 1-butyl-3-methylimidazolium chloride (BMIMCl). This will provide an ideal platform to solubilise these kinds of polymers to achieve the dissolution. The current study dissolved ...

Chitosan incorporated in the aqueous subphase is found to affect the Langmuir monolayers of cholesterol, causing the surface pressure and the surface potential isotherms to become more expanded. The mean molecular area per cholesterol molecule in the condensed monolayer increases from 53 Å in the absence of chitosan to 61 Å for a concentration of 0.100 mg/mL of chitosan in the subphase. If addi...

BACKGROUND Chitosan is a functional biopolymer that has been widely used as a hemostat. Recently, its efficacy has been questioned due to clinical failures as a result of poor adhesiveness. The purpose of this study was to compare, in a severe groin injury model in swine, the hemostatic properties of an unmodified standard chitosan sponge with standard gauze dressing and a novel hydrophobically...

This study attempted to develop chitosan-based nanoparticles with increased stability and antibacterial activity. The chitosan/protamine hybrid nanoparticles were formed based on an ionic gelation method by mixing chitosan with protamine and subsequently cross-linking the mixtures with sodium tripolyphosphate (TPP). The effects of protamine on the chemical structures, physical properties, and a...

The study describes chitosan membrane platform modified with nanostructure pattern which using nanotechnology to fabricate. The cell-substrate interaction between neuro-2a neuroblasts cell lines and chitosan membrane (flat, nanostructure and nanostructure pattern types) was investigated. The adhered morphology of neuro-2a cells depends on the topography of chitosan surface. We have found that n...

Chitosan, a natural biopolymer composed of a linear polysaccharide of α (1-4)-linked 2-amino 2-deoxy β-D glucopyranose was synthesized by deacetylation of chitin, which is one of the major structural elements, that forms the exoskeleton of crustacean shrimps. The present study was undertaken to prepare chitosan from shrimp shell waste. The physiochemical properties like degree of deacetylation ...

Global fitness analysis makes use of a genomic library of tagged deletion strains. We used this approach to study the effect of chitosan, which causes plasma membrane stress. The data were analyzed using T-profiler, which was based on determining the sensitivities of groups of deletion strains to chitosan, as defined by Gene Ontology (GO) and by genomic synthetic lethality screens, in combinati...

A new method was developed for production of low molecular weight chitosan, in which high molecular weight chitosan was treated with dilute sulfuric acid at 120°C. Chitosan was dissolved in the acid solution in a few minutes, and as depolymerized to low molecular weight chitosan by longer times. Low molecular weight chitosan was recovered from the acid by cooling down the solution and increasin...