Accurate quantification of pigments in mixtures is essential in all cases in which separation of pigments by chromatography is impracticable for one reason or another. An example is the analysis of in vivo formation of heavy metal-substituted chlorophylls in heavy metal-stressed plants. We describe here a novel, accurate UV/VIS spectrophotometric method for the quantification of individual chlo...

Chlorophyll b was first detectable after 10 minutes of illumination of etiolated pea seedlings (Pisum sativum L. var Greenfeast) with continuous white light. The chlorophyll a/b ratio decreased from 300 at 10 minutes to 15 after 1 hour. There was little change in the chlorophyll a/b ratio between 1 and 2 hours, and it declined to 3 between 2 and 5 hours of illumination. In red light, the time c...

From the absorption spectra and the excitation spectra of chlorophyll a fluorescence measured (4-77 °K) at different wavelengths (685-760 nm) and their comparison with the emission spectra published elsewhere 23, we confirm that both the Pigment Systems (I and II) contain chlorophyll b, chlorophyll a 670 and chlorophyll a 678. But System II is relatively enriched in chlorophyll b and System I i...

to comparison the photosynthetic parameters and leaf chlorophyll content and fluorescence of safflower (carthamus tinctorios l.) cultivars under salinity stress, a pot experiment was carried out as factorial based on completely randomized design with three replications in summer 2011 in university of yasouj. first factor included four salinity levels (zero, 75, 150 and 225 mm (formed as 20 to 1...

The organization of pigment molecules in photosystems is strictly determined. The peripheral antennae have both chlorophyll a and b, but the core antennae consist of only chlorophyll a in green plants. Furthermore, according to the recent model obtained from the crystal structure of light-harvesting chlorophyll a/b-protein complexes II (LHCII), individual chlorophyll-binding sites are occupied ...

Impact of the wheat stem sawßy, Cephus cinctus Norton (Hymenoptera: Cephidae), feeding injury on chlorophyll content and photosystem II (PSII) photochemistry in heads of wheat, TriticumaestivumL., at the grain-Þlling developmental stagewas evaluated by biochemically assessing the total chlorophyll, chlorophyll a (Chla), chlorophyll b (Chlb), chlorophyll a/b ratio (Chla/b), and carotenoid concen...

Illumination of 3 day old etiolated radish seedlings with continuous white light results in a progressive accumulation of chlorophyll a and b. Both pigments are bound in a different way to the thylakoid chlorophyll-proteins, which appear parallel to the formation of chlorophylls. By applying the SDS-PAGE method to SDS-digested chloroplasts, it was possible to show that the chloroplasts of radis...

Trimeric (bT) and monomeric (bM) light-harvesting complex II (LHCII) with a chlorophyll a/b ratio of 0.03 were reconstituted from the apoprotein overexpressed in Escherichia coli. Chlorophyll/xanthophyll and chlorophyll/protein ratios of bT complexes and 'native' LHCII are rather similar, namely, 0.28 vs 0. 27 and 10.5 +/- 1.5 vs 12, respectively, indicating the replacement of most chlorophyll ...

This work represents an application of the Visible Spectrometry, High Pressure Liquid Cromatography and Electronspray Ionisation-Mass Spectrometry analysis for the identification of chlorophyll and its derivatives, pheophytin and chlorophyllide, in their purified mixtures (the chlorophyll, pheophytin and chlorophyllide fraction, respectively). The chlorophyll, pheophytin and chlorophyllide frac...