We have modified a spectrometer for the measurement of fluorescence-detected magnetic (and natural) circular dichroism (FDMCD). The instrument can be operated either in a direct mode in which the average and polarization-induced differential fluorescent intensities are recorded separately or in a mode which records their ratio. We have measured the FDMCD of tryptophan, which is itself fluoresce...

A user-friendly website for the analysis of protein secondary structures from Circular Dichroism (CD) and Synchrotron Radiation Circular Dichroism (SRCD) spectra has been created.

Negative real parts of the permittivity and permeability lead an isotropic chiral medium to exhibit circular dichroism that is reversed with respect to that exhibited by an identical medium but the real parts of whose permittivity and permeability are positive.

The identification of a universal biosignature that could be sensed remotely is critical to the prospects for success in the search for life elsewhere in the universe. A candidate universal biosignature is homochirality, which is likely to be a generic property of all biochemical life. Due to the optical activity of chiral molecules, it has been hypothesized that this unique characteristic may ...

The detailed electronic structures of fluorescent chromophores are important for their use in imaging of living cells. A series of green fluorescent protein chromophore derivatives is examined by magnetic circular dichroism (MCD) spectroscopy, which allows the resolution of more bands than plain absorption and fluorescence. Observed spectral patterns are rationalized with the aid of time-depend...

Influence of chirality on the optical second harmonic generated from planar array of G-shaped metamaterials is studied. Circular dichroism of these nanostructures manifests itself via different efficiency of left and right circularly polarized second harmonic that is observed for the samples of different handedness. This difference allows to distinguish between the two enantiomers.

In this study we have showed combined effect of two single point mutations S311C (LipR2) and R214C (LipR3) on the protein stability and overall change in biochemical properties. We found that both of these mutations are near the surface and individually enhanced the thermal stability of the protein (T1/2 for S311C=4.5 h & R214C=7 h at 60°C). But, their combined effect was not additive on thermo...