WRKY proteins are an important group of transcription factors (TFs) gene family and were identified primarily in plants. TFs play vital roles modulating expression when plants face detrimental effects due to the environment. In current study, we focused on using mandarin citrus (Citrus reticulata) genome understand impact extraction alleles mining mandarins. The C. reticulata 46 CrWRKY genes th...

Diaphorina citri Kuwayama (Hemiptera: Psyllidae) is an important worldwide pest of citrus that vectors bacteria (Candidatus Liberibacter spp.) responsible for huanglongbing (citrus greening disease). We examined the behavioral responses of mated and unmated D. citri of both sexes to odors from host plants in a Y-tube olfactometer, with and without visual cues. The host plants tested were 'Dunca...

Citrus huanglongbing (HLB or citrus greening) is one of the most devastating diseases of citrus worldwide. The disease is caused by Gram-negative, phloem-limited α-proteobacterium, 'Candidatus Liberibacter asiaticus', vectored by the psyllid, Diaphorina citri Kuwayama. Citrus plants infected by the HLB bacterium may not show visible symptoms sometimes for years following infection and non-unifo...

Pectin plays a major role in pharmaceutical and food industries. To date, conventional heating method was employed for pectin extraction which time-consuming method. Therefore, this study reports the effects of conditions such as microwave power (320-640 Watt), irradiation time (10-20 seconds), dilution factor (1:10 to 1:30 weight/volume) on yield from Citrus medica peels were investigated usin...

Ruminal inoculum enriched with particle-associated microorganisms was collected from rumen cannulated Santa Inês wethers grazing tropical grass pasture and a supplement based on ground corn and soybean before feeding and used to evaluate effects of some commercial essential oils (EO) on ruminal fermentation and methane emission in vitro. The investigated EOs were orange EO (Citrus sinensis, CrS...

Background & Aim: Investigating the possibility of using medicinal plant’s seeds substitute for agar gel in plant tissue culture and determining the optimal practical conditions of them is the aim of this study. Experimental: In order to study the new gelling agents for using in plant tissue culture, we designed a tow part experiment by means of the seeds of medicinal plants such as Linum ...

Plant tryptophan decarboxylase (TDC) converts tryptophan into tryptamine, precursor of indolealkylamine alkaloids. The recent finding of tryptamine metabolites in Citrus plants leads to hypothesize the existence of TDC activity in this genus. Here, we report for the first time that, in Citrus x limon seedlings, deuterium labeled tryptophan is decarboxylated into tryptamine, from which successiv...

In citrus blight, a decline disorder of unknown etiology, the tree canopy exhibits symptoms of Zn deficiency while Zn accumulates in the trunk phloem. We have purified a Zn-binding protein (ZBP) from phloem tissue of healthy and blight-affected citrus (Citrus sinensis [L.] Osbeck on Citrus jambhiri [L.]). The molecular weight of the ZBP was estimated to be 5000 by size-exclusion chromatography ...