background the 30 kda major secretory protein of mycobacterium tuberculosis (antigen 85b) is a primary vaccine candidate. this secreted antigen induces a protective immune response and stimulates the production of ifn-γ in animal models. objectives the aim of this study was cloning and expression of ag 85b of m. tuberculosis in escherichia coli. materials and methods to produce recombinant ag85...

introduction: neurotrophins, as a family of proteins, are responsible for induction of the survival, development, and function of neurons. also, neurotropic factors are growth factors like neurotrophins that help neurons survive. moreover, neurotrophins differentiate between progenitor cells so that neurons are formed. despite the fact that the majority of mammalian brain neurons are produced p...

Attempts at cloning a quantum system result in the introduction of imperfections in the state of the copies. This is a consequence of the no-cloning theorem, which is a fundamental law of quantum physics and the backbone of security for quantum communications. Although perfect copies are prohibited, a quantum state may be copied with maximal accuracy via various optimal cloning schemes. Optimal...

The seamless ligation cloning extract (SLiCE) method is a novel seamless DNA cloning tool that utilizes homologous recombination activities in Escherichia coli cell lysates to assemble DNA fragments into a vector. Several laboratory E. coli strains can be used as a source for the SLiCE extract; therefore, the SLiCE-method is highly cost-effective.The SLiCE has sufficient cloning ability to supp...

In previous work we presented an algorithm for cloning parallel simulations that enables multiple simulated execution paths to be explored simultaneously. The method is targeted for parallel discrete event simulators that provide the simulation application developer a logical process (LP) execution model. The cloning algorithm gains efficiency by cloning logical processes only as necessary. In ...

Fictional filmic representations of human cloning have shifted in relation to the 1997 announcement of the birth of Dolly the cloned sheep, and since therapeutic human cloning became a scientific practice in the early twentieth century. The operation and detail of these shifts can be seen through an analysis of the films The Island (2005) and Aeon Flux (2005). These films provide a site for the...

We present the universal cloning transformation of states in arbitrary-dimensional Hilbert spaces. This unitary transformation attains the optimal fidelity of cloning as specified by Werner [Phys. Rev. A 58, 1827 (1998)]. With this cloning transformation, pure as well as impure states can be optimally copied, and the quality of the copies does not depend on the state being copied. We discuss th...

We propose a quantum cloning machine, which clones a qubit into two clones assuming known modulus of expectation value of Pauli z matrix. The process is referred to as the mirror phase-covariant cloning for which the input state is a priori less known than that for the standard phase-covariant cloning. Analytical expressions describing the cloning transformation and fidelity of the clones are f...

Every plant propagated by cutting is a clone of the original. Thus cloning of plants has been done since antiquity. Cloning of animals is something new. This paper reviews recent experiments with cloning of animals.