This article addresses the question of whether human reproductive cloning could be ethically justifiable in at least some cases involving infertile couples who would choose cloning as a way to have a genetically related child. At present, the risk of congenital anomalies constitutes a compelling argument against human reproductive cloning. The article explores whether reproductive cloning could...

BACKGROUND Molecular cloning is an essential step in biological engineering. Methods involving megaprimer-based PCR of a whole plasmid are promising alternatives to the traditional restriction-ligation-based molecular cloning. Their widespread use, however, is hampered by some of their inherent characteristics, e.g., linear amplification, use of self-annealing megaprimers and difficulty with pe...

muslim scholars and islamic official institutions consider human cloning as opposing religious doctrine, and forbidden. their ideas are based upon some theological and juridical arguments, including: posing a challenge to the creative power of god, breaking the tradition of marriage, breaking the tradition of diversity in creatures or species, making changes in divine creatures, game with creat...

In this study, the microbial characteristics of anaerobic reactor a sugar industry wastewater treatment plant were analyzed using cloning, FISH (Fluoresan in situ hybridization) and metagenomic analysis. Samples obtained from seven different ports on 148th day operation. The temperature was maintained at mesophilic conditions. system’s pH range operated 6.8. cloning results showed that most bac...

In this work, we design a deleting machine and shown that for some given condition on machine parameters, it gives slightly better result than P-B deleting machine [5,6]. Also it is shown that for some particular values of the machine parameters it acts like Pati-Braunstein deleting machine. We also study the combined effect of cloning and deleting machine, where at first the cloning is done by...

One of the most basic techniques in biomedical research is cDNA cloning for expression studies in mammalian cells. Vaccinia topoisomerase I-mediated cloning (TOPO cloning by Invitrogen) allows fast and efficient recombination of PCR-amplified DNAs. Among TOPO vectors, a pcDNA3.1 directional cloning vector is particularly convenient, since it can be used for expression analysis immediately after...

No molecular cloning technique is considered universally reliable, and many suffer from being too laborious, complex, or expensive. Restriction-free cloning is among the simplest, most rapid, and cost-effective methods, but does not always provide successful results. We modified this method to enhance its success rate through the use of exponential amplification coupled with homologous end-join...

Asexual reproduction in larvae, larval cloning, is a recently recognized component of the complex life histories of asteroids. We compare DNA sequences of mitochondrial tRNA genes (Ala, Leu, Asn, Pro, and Gln) from larvae in the process of cloning collected in the field with sequences from adults of known species in order to identify asteroid taxa capable of cloning. Neighbor-joining analysis i...

Perfect cloning of unknown quantum systems is forbidden by the laws of quantummechanics [1]. However, a universal optimal cloning has been proposed [2], which has been proved to be optimal in terms of fidelity [3, 4]. A obvious relevant application of such optimal cloning is eavesdropping in quantum cryptography [5]. However, quantum cloning can be of practical interest as a tool to engineer no...

The precise assembly of specific DNA sequences is a critical technique in molecular biology. Traditional cloning techniques use restriction enzymes and ligation of DNA in vitro, which can be hampered by a lack of appropriate restriction-sites and inefficient enzymatic steps. A number of ligation-independent cloning techniques have been developed, including polymerase incomplete primer extension...