Background: Tuberculosis (TB) remains as a major cause of death around the world. Construction of a new vaccine against tuberculosis is an effective way to control it. Several vaccines against this disease have been developed. The aim of the present study was to cloning of tb10.4 gene in pcDNA3.1+ plasmid and evaluation of its expression in eukaryotic cells. ...

We present a fast, reliable and inexpensive restriction-free cloning method for seamless DNA insertion into any plasmid without sequence limitation. Exponential megapriming PCR (EMP) cloning requires two consecutive PCR steps and can be carried out in one day. We show that EMP cloning has a higher efficiency than restriction-free (RF) cloning, especially for long inserts above 2.5 kb. EMP furth...

UNLABELLED This research focused on understanding the attitudes toward human cloning in Taiwan among professionals in healthcare, law, and religion. DESIGN The study was conducted utilizing a structured questionnaire. PARTICIPANTS 220 healthcare professionals from two regional hospitals located in Taipei, 351 religious professionals in the northern Taiwan and 711 legal professionals were se...

Abstract At present, many cloning selection algorithms have been studied, and improvements made to the cloning, mutation steps. However, there is a lack of research on optimization updating operation The clonal algorithm traditionally updated through random complement antibodies, which blind uncertain process. added antibodies may gather near local optimal solution, resulting in need for more i...

Abstract There can be many reasons for anyone to make a digital copy (clone) of own decision-making behavior. This enables virtual presence professional decision-maker simultaneously in places and processes Industry 4.0. Such clone used as one’s responsible representative when the human is not available. Pi-Mind (“Patented Intelligence”) technology, which “cloning” cognitive skills humans using...

Numerous uncertainties are hanging over the biotechnology of human cloning which has prompted medical ethicists and religious organizations to ask questions that bordered on its ethical considerations. In humans, issues arise both in clinical laboratory settings hence, morality manipulating genes is foremost issue among scientists scholars. Therefore, this paper evaluated technology using perso...

We introduce the concept of cloning for classes of observables and classify cloning machines for qubit systems according to the number of parameters needed to describe the class under investigation. A no-cloning theorem for observables is derived and the connections between cloning of observables and joint measurements of noncommuting observables are elucidated. Relationships with cloning of st...

We have developed an efficient strategy for cloning of PCR products that contain an unknown region flanked by a known sequence. As with ligation-independent cloning, the strategy is based on homology between sequences present in both the vector and the insert. However, in contrast to ligation-independent cloning, the cloning vector has homology with only one of the two primers used for amplific...

objective(s): hepatitis c is a major cause of liver failure worldwide. current therapies applied for this disease are not fully effective and produce side effects in most cases. non-structural protein 3 helicase (ns3) of hcv is one of the key enzymes in viral replication and infection. therefore, this region is a promising target to design new drugs and therapies against hcv infection. the aim ...