Ser/thr phosphatases dephosphorylate their targets with high specificity, yet the structural and sequence determinants of phosphosite recognition are poorly understood. Calcineurin (CN) is a conserved Ca(2+)/calmodulin-dependent ser/thr phosphatase and the target of immunosuppressants, FK506 and cyclosporin A (CSA). To investigate CN substrate recognition we used X-ray crystallography, biochemi...

Transient electronic and vibrational absorption spectroscopy unravel the mechanisms and dynamics of bimolecular reactions of CN radicals with acetone in deuterated chloroform solutions. The CN radicals are produced by ultrafast ultraviolet photolysis of dissolved ICN. Two reactive forms of CN radicals are distinguished by their electronic absorption bands: "free" (uncomplexed) CN radicals, and ...

Cationorm® (CN) cationic nanoemulsion was demonstrated to enhance tear film (TF) stability in vivo possibly via effects on tear film lipid layer (TFLL). Therefore the interactions of CN with human meibum (MGS) and TFLL in vitro and in vivo deserve special study. MGS and CN were spread at the air/water interface of a Langmuir surface balance to ensure a range of MGS/CN oil phase ratios: 20/1, 10...

Hydrogenasen sind H2-umsetzende Metalloenzyme und enthalten katalytische Kofaktoren, deren Eisenionen durch biologisch ungewöhnliche Kohlenmonoxid- (CO) Cyanid- (CN−) Liganden koordiniert sind. Externes CO CN−hemmt jedoch. Der molekulare Mechanismus der Bindung von CN− an [FeFe]-Hydrogenasen ist unbekannt. In dieser Studie präsentieren wir Kristallstrukturen mit behandelten [FeFe]-Hydrogenase C...

We investigated the role of tumor copy number (CN)-altered genome (CN-AG) in the carcinogenesis of cervical cancer (CC), especially its effect on gene expression, biological processes, and patient survival. Fifty-nine human papillomavirus 16 (HPV16)-positive CCs were investigated with microarrays-31 for mapping CN-AG and 55 for global gene expression, with 27 CCs in common. Five-year survival w...

The catalytic capability of original carbon nitride (CN) is limited by a small specific surface area and high electron–hole recombination rate. In this study, WO3-loaded porous nanosheets (MCA-CN/WO3) were synthesized thermal treatment with melamine, cyanuric acid WCl6. MCA-CN/WO3 could degrade 98% the methylene blue (MB) within 30 min in photo-Fenton-like process, displaying better activity th...

Discrete nonlinear Schröginger equation (DNLS) of the form, in dt = Cn+1 + Cn−1 χn[|Cn+1| + |Cn−1| − 2|Cn|]Cn is used to study the formation of stationary localized states in one dimensional system due to a single as well as a dimeric nonlinear impurity. The fully nonlinear chain is also considered. The stability of the states and its connection with the nonlinear strength is presented. Results...

Layered copper-nickel cyanide, CuNi(CN)4, a 2-D negative thermal expansion material, is one of a series of copper(ii)-containing cyanides derived from Ni(CN)2. In CuNi(CN)4, unlike in Ni(CN)2, the cyanide groups are ordered generating square-planar Ni(CN)4 and Cu(NC)4 units. The adoption of square-planar geometry by Cu(ii) in an extended solid is very unusual.

(1) MSc., Delft University of Technology, Stevinweg 1, Delft, 2628 CN, The Netherlands. [email protected] (2) MSc., Delft University of Technology, Stevinweg 1, Delft, 2628 CN, The Netherlands. [email protected] (3) MSc., Delft University of Technology, Stevinweg 1, Delft, 2628 CN, The Netherlands. [email protected] (4) PhD. MSc., Delft University of Technology, St...