Resonance Raman and absorption spectra were examined for the species generated upon incubation of oxidized bovine cytochrome c oxidase at pH 8.0 with carbon monoxide in the presence or absence of O2. The anaerobic incubation yielded the CO isotope sensitive bands at 517 and 578 cm–1 for 12C16O, at 512 and 563 cm–1 for 13C16O, at 508 and 574 cm–1 for 12C18O, and at 503 and 557 cm–1 for 13C18O, i...

Granulocytes play a key role in the body's innate immune response to bacterial and viral infections. While methods exist to measure granulocyte function, in general these are limited in terms of the information they can provide. For example, most existing assays merely provide a percentage of how many granulocytes are activated following a single, fixed length incubation. Complicating matters, ...

It is generally considered that both w and i3-adrenoceptors exist in rat parotid glands (1, 2). In our previous paper, the effect of adrenergic stimuli on spontaneous potassium fluxes was found to be distinctly different between at and i3-adrenergic agonists, the former increases potassium efflux and the latter decreases potassium release (3, 4). The present in vitro investigation was undertake...

background: in the field of mammalian embryo culture, the putative influence of autocrine/ paracrine factor(s), produce by the embryos itself, is under investigation. a smaller medium drop can prevent dilution of this factor(s). objective: the objective of this study was to examine the effect of culture medium volume on in vitro development of mouse 2-cell embryos. materials and methods: the em...