The aim of the study was to characterise the 493 tube-coagulase-negative Staphylococcus sp. strains isolated from the udder quarters of milking dairy cows in one herd with a high bulk milk somatic cell count (SCC). The isolates were examined phenotypically by cultural features, Gram stain, catalase, coagulase, and API biochemical tests, and genotypically by PCR for chosen genes. About 10% of th...

In August 2005, there was an outbreak of food poisoning in a hotel under the jurisdiction of the Minamikaga Health Center, Ishikawa Prefecture, Japan. The health center’s investigation revealed that there were a total of 10 infected individuals. Two to 12 h (average 7 h) after the dinner that was considered responsible for the food poisoning, the patients developed diarrhea (100%), abdominal pa...

OBJECTIVES Staphylococcus aureus is an important bacterial pathogen responsible for a variety numbers of nosocomial and community acquired infections. Biofilm formation is regarded as an important factor in the establishment of S. aureus infection. The contribution of the genetic background of S. aureus to biofilm formation is poorly understood. The aim of the present work was to genotype S. au...

The SaeRS two-component system plays important roles in regulation of key virulence factors and pathogenicity. In this study, however, we found that the deletion mutation of saeRS enhanced bacterial survival in human blood, whereas complementation of the mutant with SaeRS returned survival to wild-type levels. Moreover, these phenomena were observed in different MRSA genetic background isolates...

OBJECTIVES Integrons are thought to play an important role in the spread of antibiotic resistance. This study investigates class 1 and 2 integron-positive methicillin-resistant coagulase-negative staphylococci strains isolated in Iran and characterizes their patterns of antimicrobial resistance. METHODS Hundred clinical isolates of coagulase-negative staphylococci were characterized for integ...

results of total 60 s. aureus isolates, 51 (85%) were mrsa, and of 60 cons, 7 (11.66%) were mrcons. the results showed that the mic of chlorhexidine for all 120 isolates was 1-16 μg/ml. 15 (12.5%) isolates carried qaca/b gene, 26 (21.7%) carried qacc gene, and 38 (31.7%) carried smr gene. conclusions maintenance of mrsa isolates in the attendance of low amounts of antiseptics could result in th...

Background: The present study is the first comprehensive report of the Molluscum contagiosum virus (MCV) in Iran based on the molecular technique for differentiation and typing of the MCV1 and MCV2. Methods: Patients were diagnosed as having tumor-like genital warts less than 5 mm in diameter, and HIV seronegative samples were chosen for this cross-sectional study. TaqMan real-time PCR was used...

BACKGROUND Otitis externa is a common inflammatory ear disease in dogs caused by a variety of pathogens, and coagulase-positive staphylococci are frequently isolated from such infections. OBJECTIVE To identify antimicrobial susceptibility profiles and methicillin-resistant strains among coagulase-positive staphylococci isolated from otitis externa in dogs. METHODS A cross-sectional study wa...

The relative frequency of 10 determinants of resistance to macrolides, lincosamides, and streptogramins was investigated by PCR in a series of 294 macrolide-, lincosamide-, and/or streptogramin-resistant clinical isolates of Staphylococcus aureus and coagulase-negative staphylococci isolated in 1995 from 32 French hospitals. Resistance was mainly due to the presence of ermA or ermC genes, which...