Over the past decade, biodiversity researchers have dedicated tremendous efforts to constructing DNA reference barcodes for rapid species registration and identification. Although analytical cost for standard DNA barcoding has been significantly reduced since early 2000, further dramatic reduction in barcoding costs is unlikely because Sanger sequencing is approaching its limits in throughput a...

Descriptions are given of three new cryptic species of Merodon Meigen (Diptera: Syrphidae) from the island of Lesvos (Greece): Merodon latifemoris Radenković et Vujić n. sp. from the nigritarsis species group, Merodon pulveris Vujić et Radenković n. sp. from the natans species group and Merodon puniceus Vujić, Radenković et Pérez-Bañón n. sp. from the aureus species group. In addition to classi...

In this study we report on the sequencing of the COI barcode region from 96 historical specimens (92 type specimens +4 non-types) of Eois. Eois is a diverse clade of tropical geometrid moths and is the target of a number of ongoing studies on life-histories, phylogeny, co-evolution with host plants or parasitoids, and diversity patterns across temporal and spatial dimensions. The unequivocal ap...

Aiming at the 2D color barcode images captured by mobile phone camera under complex environment, this paper presents a novel segmentation algorithm of 2D color barcode image based on gradient features. Firstly, the gradient image can be obtained by extracting gradient features from the image captured. Then the DCT transformation algorithm is used to partition the gradient image and the enhanced...

DNA barcoding has revolutionized how we discover, identify, and detect species. A substantial foundation been established with millions of mitochondrial cytochrome c oxidase I sequences freely available for eukaryotes. However, issues COI ranging from uniparental inheritance small genetic population sizes to nuclear asymmetric introgression can impede its use. We propose using CAD as the “nucle...

Partial (∼ 780 bp) mitochondrial cytochrome c oxidase subunit I (COI) and near complete nuclear 18S rDNA (∼ 1,780 bp) sequences were directly compared to assess their relative usefulness as markers for species identification and phylogenetic analysis of coccidian parasites (phylum Apicomplexa). Fifteen new COI partial sequences were obtained using two pairs of new primers from rigorously charac...

BACKGROUND DNA barcoding aims to provide an efficient method for species-level identifications using an array of species specific molecular tags derived from the 5' region of the mitochondrial cytochrome c oxidase I (COI) gene. The efficiency of the method hinges on the degree of sequence divergence among species and species-level identifications are relatively straightforward when the average ...

Because sequence information is now available for the 648bp barcode region of cytochrome c oxidase 1 (COI) from more than 400,000 animal species, this gene segment can be used to probe patterns of mitochondrial evolution. The present study examines levels of amino acid substitution and the frequency of indels in COI from 4177 species of arachnids, including representatives from all 16 orders an...

The DNA barcode of Bruchidius varius collected from Dilek Peninsula and Büyük Menderes Delta National Park (DPNP) in Aydın province Turkey is given for the first time. As barcode, mitochondrial cytochrome c oxidase subunit I (COI) with a length approximately 628 bp sequenced an illustrative presented along nucleotide composition target gene location. Nucleotide sequence includes 38.63% T, 27.98...

The present study represents a comprehensive molecular assessment of some family of freshwater fishes in Iran. We analyzed cytochrome oxidase I (COI) sequences for five genus of cypriniform fishes from Iran. The present investigation provides data on genetic structure of some species of Nemachilidae including Paraschistura bampurensis, Oxynoemacheilus kiabii and Turcinemacheilus saadii and Leuc...