BACKGROUND Estimation of urinary oxalate is one of important tools for the diagnosis and treatment of urolithiasis. And more precise, simple and inexpensive method is desirable. In the present study, we evaluated three methods which were clinically well-used. METHODS From October 1996 to June 1997, 146 acidified urine samples were collected for 24 hours from 144 urolithiasis patients. We dete...

A colorimetric method for quantitative measurement of the susceptibility of Mycobacterium tuberculosis to antimicrobial agents is described. The method utilizes an oxidation-reduction dye, Alamar blue, as an indicator of growth. By this method, MICs of isoniazid, rifampin, streptomycin, and ethambutol were determined for 50 strains of M. tuberculosis. Colorimetric MIC results were available on ...

In the estimation of very small q-qantities of material there is no question that colorimetric is preferable to gravimetric analysis. The colorimetric method is generally quicker and simpler to carry out-an important factor when numerous analyses are involvedand it is not subject to the manipulative errors incurred in dealing gravimetrically with minute amounts of material. The method, however,...

MicroRNAs are small regulatory molecules that can be used as potential biomarkers of clinical diagnosis, and efforts have been directed towards the development of a simple, rapid, and sequence-selective analysis of microRNAs. Here, we report a simple and versatile colorimetric strategy for ultrasensitive and specific determination of microRNAs based on molecular beacon initiated strand displace...

Factors and mechanisms controlling lipometabolism homeostasis share a remarkable evolutionary conservation between humans and Drosophila flies. Accordingly, the Drosophila model has been successfully used to understand the pathophysiology of human metabolic diseases such as obesity. Body fat stores in species as different as humans and flies consist of neutral lipids, mainly triacylglycerols. C...

This study aimed to examine the differences in the morphological properties and proliferation of olfactory ensheathing cells in three-dimensional culture on collagen-heparan sulfate biological scaffolds and in two-dimensional culture on common flat culture plates. The proliferation rate of olfactory ensheathing cells in three-dimensional culture was higher than that in two-dimensional culture, ...

A new highly selective and sensitive colorimetric chemosensor 1 for Co(2+) was developed. The receptor 1 sensed Co(2+) by changing its color from yellow to orange in aqueous solution. Moreover, 1 could be used as a practical, visible colorimetric test kit for Co(2+).

The synthesis of a novel aza-BODIPY dye functionalized with fused pyrazine rings, suitable for use as a selective colorimetric and fluorometric sensor for NH(4)(+), is outlined. In addition to significant fluorescence quenching, an obvious colorimetric change from green to red-pink is observed enabling facile "naked-eye" detection of NH(4)(+).

The objective of the present work was to compare colorimetric and distillationÈtitration methods to determine the ammonia-nitrogen con3 centration in sheep and goat urine samples. Colorimetric methods used were based on the indophenol reaction, whereas titrimetric methods were based on the alkali distillation of ammonia from the urine and its titrimetric determination with a standard acid. Colo...