conclusions genome instability in addicted patients was demonstrated in this study. controversially, considering incoherent results of previous studies and our data, more studies in longer duration of methadone use are needed to elucidate the consequence. results the mutagenicity index in addicted group was robustly higher than healthy volunteers. fortunately, this significant difference was lo...

background the increasing use of herbal drugs and their easy availability have necessitated the use of mutagenicity tests to analyze their toxicity and safety. objectives the aim of this study was to determine the in vitro mutagenicity of artemisia draconculus l., a herbal drug, by performing single cell gel electrophoresis (scge). materials and methods in this study, we obtained a herbal drug ...

In this paper, we discuss the different strategies used in COMET (COordinated Multimedia Explanation Testbed) for selecting words with which the user is familiar. When pictures cannot be used to disambiguate a word or phrase, COMET has four strategies for avoiding unknown words. We give examples for each of these strategies and show how they are implemented in COMET.

The Stardust spacecraft collected thousands of particles from comet 81P/Wild 2 and returned them to Earth for laboratory study. The preliminary examination of these samples shows that the nonvolatile portion of the comet is an unequilibrated assortment of materials that have both presolar and solar system origin. The comet contains an abundance of silicate grains that are much larger than predi...

In this work, the DNA-damaging effect of hydrogen peroxide on the structural integrity of nucleolar organizer regions (NORs) was studied for the first time by comet-FISH in the Pacific oyster Crassostrea gigas. Global DNA damage was assessed in hemocytes using an alkaline version of the comet assay. Next, NOR sensitivity was analyzed by mapping major rDNA repeat unit by fluorescence in situ hyb...

Learning distance metrics from data is a fundamental problem in machine learning and useful way to extract data-driven features by using the matrix root of a distance matrix. Finding a proper metric amounts to optimization over the cone of positive definite (PD) matrices. This optimization is difficult since restricting optimization to remain within the PD cone or repeatedly projecting to the c...

The electrophoresis pattern of apoptotic cells detected by the comet assay has a characteristic small head and spread tail. This image has been referred to as an apoptotic comet, but it has not been previously proven to be apoptotic cells by any direct method. In order to identify this image obtained by the comet assay as corresponding to an apoptotic cell, the frequency of appearance of apopto...

Polymerization of actin filaments is necessary for both protrusion of the leading edge of crawling cells and propulsion of certain intracellular pathogens, and it is sufficient for generating force for bacterial motility in vitro. Motile intracellular pathogens are associated with actin-rich comet tails containing many of the same molecular components present in lamellipodia, and this suggests ...

The comet assay has been widely used to measure a range of cellular responses to DNA damage and has found applications in genotoxicity studies, bio-monitoring, ecological testing and in the study of human disease. This review discusses how the comet assay has been applied to the study of DNA damage and repair associated with cancer. The potential of the assay as a tool for predicting an individ...