BACKGROUND The aim of this study was to compare invasive and non-invasive strains of Shigella flexneri isolated from Tehran by a 120 kDa protein band by SDS-PAGE, electron microscopy of cell culture and Congo red dye methods. METHODS S. flexneri strains were isolated by standard bacterial methods from fecal specimens of children attending to the 3 children's hospitals. Phenotype analysis for ...

A Congo red-magnesium oxalate agar medium was developed to detect expression of virulence-associated calcium dependency and Congo red absorption in Yersinia enterocolitica. Of the 157 pathogenic serotypes tested, 119 (75.8%) were positive; 98% of nonpathogenic serotypes and strains of three other Yersinia species were negative.

background: tumor associated tissue eosinophilia is believed to play a significant role in the biological behavior of the carcinoma. eosinophil infiltrate in association with the head and neck squamous cell carcinoma (scc) have been reviewed from time-to-time. the significance of such an association has been variably thought to be either a potential diagnostic tool for stromal invasion or as a ...

OBJECTIVE To screen for amyloid and to assess associated clinical and laboratory characteristics in Egyptian patients with rheumatoid arthritis (RA). METHODS Abdominal subcutaneous fat aspirates were consecutively collected from 112 patients (103 women, nine men) having RA for five years or more. To detect amyloid, fat smears were stained with Congo red and the concentration of amyloid A prot...

In this work, we present the stabilization of polar step edges along the [010] direction of calcite (10.4) by the presence of a water-soluble organic molecule, namely Congo Red. While characteristic etch pits are observed on the surface in the absence of the additive, no etch pits can be found in the presence of the additive. Using atomic force microscopy, we can directly follow the restructuri...

OBJECTIVE To describe a new, quantitative, and reproducible method for detecting deposits of amyloid A protein in aspirated fat tissue and to compare it with smears stained with Congo red. METHODS After extraction of at least 30 mg of abdominal fat tissue in guanidine, the amyloid A protein concentration was measured by a monoclonal antibody-based sandwich ELISA. RESULTS The concentrations ...

This study reports decolorization of Rhodamine B and Congo red by a partially purified laccase from Lentinus polychrous Lév. in the presence or absence of 2,2-azinobis (3-ethylbenzothiazoline-6sulfonate) diammonium salt (ABTS) as a redox mediator. The decolorization study showed that about 90% of Rhodamine B could be decolorized within 52 h, whereas Congo red could be decolorized more than 75% ...

An alternative method for detecting the production of slime by coagulase negative staphylococci was compared with the routinely used Christensen method on 124 isolates ofcoagulase negative staphylococci from carriage sites, blood cultures, and infected peritoneal dialysis fluids. The alternative method requires the use ofa specially prepared solid medium-brain heart infusion broth, supplemented...

A large number of microorganisms are capable of degrading cellulose, only a few of these microorganisms produce significant quantities of enzymes capable of completely hydrolyzing cellulose. Fungi are the main cellulase producing microorganisms. In this purposed study, four fungal species belonging to two genera i.e. Trichoderma and Aspergillus were isolated from different sources, screened and...

A punch biopsy of the axillary region was performed for histopathological study, staining with hematoxylin-eosin (Figure 2), periodic acid-Schiff (PAS), and Congo red. The histological findings included papillomatosis, orthokeratotic hyperkeratosis and epidermal acanthosis, focal atrophy of the granular layer, and mild hyperpigmentation of the basal layer of the epidermis. There was a moderate ...