Background: One of the major factors affecting on male infertility is oxidative stress (OS). Two main sources of ROS production are leukocytes and spermatozoa. The ROS such as tertiary-butyl hydroperoxide (TBHP) may damage and fragmentate to sperm DNA, which can lead to complications in the offspring. In this study, we will investigate the effect of od on DNA fragmentation index (DFI) in sperm ...

Objective: In this paper, we have introduced topoisomerase inhibitors, mechanism of action and types of them. DNA topoisomerases are ubiquitous enzymes that catalyze essential enzymes to solve the topological problems accompanying key nuclear processes such as DNA replication, transcription, repair and chromatin assembly by introducing temporary single or double strand breaks in the DNA. Result...

Objective: In this paper, we have introduced topoisomerase inhibitors, mechanism of action and types of them. DNA topoisomerases are ubiquitous enzymes that catalyze essential enzymes to solve the topological problems accompanying key nuclear processes such as DNA replication, transcription, repair and chromatin assembly by introducing temporary single or double strand breaks in the DNA. Result...

Background: It is stated that pentoxifyline can improve sperm motility and suggested to use in ART for asthenozoospermia. But, probable effect of this drug on sperm DNA in unknown. Our main goal was to evaluate the impact of pentoxifyline on sperm DNA fragmentation. Materials and Methods: Thirty eight asthenozoospermic patients were selected for this prospective study. After direct swim-up, spe...

objective: mitochondrial dna (mtdna) is a useful tool for population studies, identification of humans and forensic dna studies. the existence of several hundreds copies of mtdna per cell permit its extraction from minute or degraded samples. in addition, the level of polymorphism in the hypervariable (hv) region is high enough to permit its use in human identity testing. however, the presence ...

Background: Although sperm selection according to morphologic and motility characteristic, made to some what the fertility successful, but sperm’s normal morphology cannot demonstrate sperm DNA competence. Since the health of sperm DNA is affecting the results of assisted reproductive technology; the aim of this study is to evaluate semen parameters measured by CASA with sperm chromatin abnorma...