Tailing of DNA with terminal transferase is a technically demanding, but essential step in full-length cDNA cloning procedures*•^. It is also an essential step of a poly(dA) affinity labelling technique, which has been used to select families of partial restriction fragments on oligo(dT)cellulose^•*. In order to bypass the need for transferase in the latter technique, we made a simple modificat...

Rare earth mine tailing dumps are environmental hazards because tailing easily leaches and erodes by water and wind. To assess the influence of mine tailing on the geochemical behavior of rare earth elements (REEs) in soil, sixty-seven surface soil samples and three soil profile samples were collected from different locations near China's largest rare earth mine tailing. The total concentration...

Reuse of process water in the flotation sulfide ores has crucial importance for environmental sustainability and improved economy. However, chemistry may be a critical subject efficiency as dissolved ion concentration increases with reuse. In this study, effects reuse on Cu-Zn ore were investigated. The flowsheet consists pre-flotation section to remove naturally floatable talc particles, seque...

Non-specific tail sequences are often added to the 5'-terminus of primers to improve the robustness and overall performance of diagnostic assays. Despite the widespread use of tailed primers, the underlying working mechanism is not well understood. To address this problem, we conducted a detailed in vitro and in silico analysis of the enhancing effect of primer tailing on 2 well-established foo...

RFID (Radio-Frequency IDentification) is a key emerging technology for supply-chain monitoring and detection of counterfeit and grey-market goods. The most prevalent RFID tags are, however, simply “wireless barcodes,” themselves vulnerable to cloning and counterfeiting. While continuous monitoring can, in principle, detect cloning attacks, real-world supply chains often contain significant blin...

Biological nitrogen fixing is an important source of nitrogen input in the natural ecological restoration of mine wastelands. The diversity of nifH genes in tailings samples under different plant communities in Yangshanchong and Tongguanshan wastelands in Tongling, was analyzed using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) approach. The nitrogen-fixing micro...

Utilizing cyanide compounds in mining and chemical industry is one of the most important environmental issues due to the acute toxic properties of many cyanide compounds to humans and aquatic life. Cyanide tends to react readily with most other chemical elements, producing a wide variety of toxic, cyanide related compounds. This research was aimed at investigating a feasible and economical tech...

AIM This study evaluates the effect of surface application of dried Class A biosolids on microbial populations within copper mine tailings. METHODS AND RESULTS Mine tailing sites were established at ASARCO Mission Mine close to Sahuarita Arizona. Site 1 (December 1998) was amended with 248 tons ha(-1) of Class A biosolids. Sites 2 (December 2000) and 3 (April 2006) were amended with 371 and 2...

Workability is a key performance criterion for mining cemented tailing backfill, which should be defined in terms of rheological parameters such as yield stress and plastic viscosity. Cemented tailing backfill is basically composed of mill tailings, Portland cement, or blended cement with supplementary cement material (fly ash and blast furnace slag) and water, among others, and it is important...