The aim of this study was to evaluate the performance of the RIDA® Quick Cryptosporidium/Giardia-Combi test (R-Biopharm AG) as a rapid method for the routine diagnosis of giardiasis in dogs. Compared to an ELISA coproantigen test (Prospect® Giardia Microplate Assay)), the immunochromatographic test had a sensitivity of 83 %, a specificity of 79 %, a positive predictive value of 33 %, and a nega...

BACKGROUND Emergence of human fascioliasis prompted a worldwide control initiative including a pilot study in a few countries. Two hyperendemic areas were chosen: Huacullani, Northern Altiplano, Bolivia, representing the Altiplanic transmission pattern with high prevalences and intensities; Cajamarca valley, Peru, representing the valley pattern with high prevalences but low intensities. Coprol...

Accurate diagnosis of infection with the parasite Strongyloides stercoralis is hampered by the low concentration of larvae in stool, rendering parasitological diagnosis insensitive. Even if the more sensitive agar plate culture method is used repeated stool sampling is necessary to achieve satisfactory sensitivity. In this manuscript we describe the development of a coproantigen ELISA for diagn...

In the present study the dynamics of antigenemia and coproantigens were studied in patients with Fasciola hepatica infection during an outbreak occurring in La Palma, Pinar del Río, in the West Province of Cuba. Stool and serum samples were collected from 67 patients and 40 healthy subjects. Stool samples were studied by a simple gravity sedimentation technique and an ES78 sandwich enzyme-linke...

BACKGROUND The study was targeted to report the appearance of coproantigens in feces and circulating antibodies in the serum of Fasciola gigantica experimentally infected rabbits. METHODS Copro Hyper Immune Serum (HIS) and Excretory-Secretory Hyper Immune Serum (ES HIS) antigens were used in a sandwich ELISA for the detection of F. gigantica antigens in feces of 12 rabbits experimentally infe...

We evaluated the diagnostic performance of a Cryptosporidium immunoenzymatic assay (ELISA). Fecal samples were collected from 94 HIV-seropositive patients. All specimens were processed with a commercially-available ELISA to detect C. parvum specific coproantigen and with a modified Ziehl-Neelsen stain (ZNm) microscope exam. Overall, sensitivity of the immunoenzymatic test was 100%, with a speci...

background: cryptosporidium is a protozoan parasite with worldwide distribution. the aim of this study was to estimate the prevalence of cryptosporidium infection by antigen detection in faeces among immunocompromised patients referred to educational hospitals of ahvaz city, south-west of iran, 2009-2010. methods : fecal samples from 176 immunocompromised patients were collected and cryptospori...