background: echinococcosis is one of the most important helminthic zoonotic diseases in iran. intestinal scraping tech­nique (ist), the traditional method for diagnosis of the infection in definitive hosts, has many disadvantages including low sus­ceptibil­ity and being expensive, hazardous and laborious. detection of coproantigens in fecal samples by enzyme-linked immu­nosorbent assay (ca-elis...

cystic echinococcosis is an important zoonosis in the sheep rising areas of iran. to develop a simple and reliable diagnostic method for echinococcus infection in definitive hosts, e. granulosus polyoclonal antibodies (polyabs) were prepared from adult worm in rabbit. a selected poab was used for coproantigen detection in faecal samples obtained from animals naturally infected with echinococcus...

The detection of Fasciola antigen in serum or stool could be more valuable in diagnosis, hence early treatment before irreparable damage. In this study, fresh adult Fasciola gigantica worms were collected, then incubation in culture medium and collected medium was used to extract crude excretory-secretory (E/S) antigen. E/S was used to immunize rabbits to raise specific antibodies against Fasci...

In the eastern Tibetan plateau both human cystic and alveolar echinococcosis (AE) caused by infection with Echincoccus granulosus or Echinococcus multilocularis, respectively are highly endemic. The domestic dog plays a key role in zoonotic transmission in this region. Our primary objective was to investigate the role of domestic dogs in maintaining transmission of E. multilocularis in Shiqu co...

Two fractions were isolated from coproantigen by ion-exchange chromatography in which DEAE cellulose was utilized. Both fractions and crude antigen were characterized by SDS-polyacrylamide gel electrophoresis which revealed 13 bands of molecular weight ranged from 205-31 in crude coproantigen. While fraction I resolved into six bands of molecular weight 198, 178, 148, 111, 101 & 45. Fraction II...

High prevalences of Echinococcus multilocularis have been reported from foxes of the city of Zurich, Switzerland. In order to characterize transmission in urban areas, a coproantigen ELISA was evaluated for diagnosing the infection in fox faecal samples collected in the environment. In addition, trapped rodents were investigated for the presence of metacestodes. Faecal samples could reliably be...

A survey was done in an attempt to investigate the epidemiological status of Echinococcus multilocularis in red foxes and raccoon dogs in Otaru city from June to September 1999. Sixty-seven red foxes (Vulpes vulpes schrencki) and 13 raccoon dogs (Nyctereutes procyonoides albus) were captured, and postmortem examinations were conducted with them. Thirty-eight red foxes (56.7%) and 3 raccoon dogs...

This study reports on the kinetics of antibody production to Echinostoma caproni and the dynamics of antigens in feces and sera in 2 experimental hosts (hamsters and rats) that display different degrees of susceptibility with this echinostome. Echinostoma caproni produced chronic infections in hamsters, whereas rats lost the infection at 49-56 days postinfection (DPI). Hamsters developed higher...

A large sheep-dog population from the province of Alava (northern Spain) has been investigated in order to determine the prevalence of the cestode parasite Echinococcus granulosus. Worms were detected in 14.0% of 721 dog faecal supernatants by coproantigen ELISA, and in 9.1% of 754 dog serum samples by serum antibody ELISA. A weak but statistically significant correlation (Spearman's rho=0.103,...