Commercial control preparations were exposed to light at room temperature for as long as 24 h. Decreases in creatine kinase activity in seven of the nine controls ranged from 28.8 to 83.3%. When these controls were protected from light and stored at various temperatures, the decrease in activity was either eliminated or substantially reduced, indicating that the decreased activity in the presen...

We describe a practical, technically convenient DEAE-Sephadex chromatographic column and a three-fraction salt-gradient elution procedure, with which serum creatine kinase activity is rapidly and quantitatively separated into the isoenzyme components with excellent analytical recovery of the total activity applied. The homogeneity of the isoenzyme fractions was demonstrated by electrophoresis a...

MM creatine kinase purified from tissue exists as a single form but upon release into the plasma exhibits three forms which, based on increasing anodal migration on polyacrylamide gel electrophoresis, are referred to as MM1, MM2, and MM3. The three forms were isolated in pure form from plasma in sufficient quantities for biochemical and physiological studies. Chromatofocusing results show that ...

Alterations in the competency of the creatine kinase system elicit numerous structural and metabolic compensations, including changes in purine nucleotide metabolism. We evaluated molecular and kinetic changes in AMP deaminase from skeletal muscles of mice deficient in either cytosolic creatine kinase alone (M-CK-/-) or also deficient in mitochondrial creatine kinase (CK-/-) compared with wild ...

2. Dawson DM, Fine IH. Creatine kinase in human tissue. Arch Neurol 1966;16:175-80. 3. Van Der Veen KJ, Willebrands AF. Isoenzyme of creatune phosphokunasein tissue extract and in normal and pathologic sera. Clin Chim Acts 1966;13:312-5. 4. Wagner GS, Roe CR, Liinbird LF, et al. The importanceof identificationof myocardiac specific isoenzyme of creatune phosphokinase (MB form) in the diagnosis ...

Six hundred 1-d-old male broilers (Ross 308) were assigned to four experimental groups; each was composed of 5 floor pen replications of 30 birds including control (no enalapril), 15, 30 and 60 ppm enalapril in the drinking water. From d 21 to 49, all the chicks were exposed to low ambient temperature to induce ascites. Mortalities were inspected to determine the cause of death and diagnose of ...

Aim: The aim of this paper was to determine whether; (1) patella tendon stiffness, (2) the magnitude of vastus lateralis fascicle lengthening, and (3) eccentric torque correlate with markers of exercise induced muscle damage. Method: Combining dynamometry and ultrasonography, patella tendon properties and vastus lateralis architectural properties were measured pre and during the first of six se...