Cryopreservation is becoming a very important tool for the long-term storage of plant genetic resources and efficient cryopreservation protocols have been developed for a large number of plant species. Practical procedures, developed using in vitro tissue culture, can be a simple and reliable preservation option of potato genetic resources rather than maintaining by vegetative propagation in ge...

A cryopreservation condition for D-amino acid oxidase (DAAO)-overexpressing Escherichia coli (E. coli BL21(DE3)/pET-DAAO) was established. Ten percent was the optimum concentration of glycerol as a cryoprotectant, and its diffusion into stationary phase cells was superior to that into log cells. The results also showed that rather than fast cooling, a slow cooling method was appropriate to our ...

With improved survival rates among cancer patients, fertility preservation is now being recognized as an issue of great importance. There are currently several methods of fertility preservation available in female cancer patients and the options and techniques via assisted reproduction and cryopreservation are increasing, but some are still experimental and continues to be evaluated. The establ...

the aim of this study was to determine the effects of the antioxidants superoxide dismutase (sod),glutathione peroxidase (gpx) and butylated hydroxytoluene (bht) on microscopic semen parameters andlipid peroxidation following the freeze-thawing of bull semen. ejaculates were collected from five holsteinbulls and pooled at 37°c. the semen samples were diluted with a cey extender containing addit...

the objective of this study was to evaluate quality of frozen-thawed bull semen processed with extenders containing vitamin c and e as antioxidants. pooled semen's were collected from 5 bulls and diluted to a concentration of 30 × 106 sperm/ml with citrate –egg yolk (cey) or tris – egg yolk (tey) extenders. the diluted semen was divided to 5 aliquots including control and 4 experimental groups....

objective: to evaluate the effect of honey supplemented to cryoprotectant medium on post-thaw sperm motility, concentration, morphology and agglutination. materials and methods: thirty semen samples were collected from 30 infertile patients. after assessment of semen analysis, semen samples were divided into 3 aliquots (0.7ml for each) and mixed with 1 ml of cryopreservation solution (g1, contr...

Despite the significant progress, the post-thaw viability and fertility of the cryopreserved sperm are still reduced, as a consequence of accumulated cellular injuries that arise throughout the cryopreservation process. Many laboratory tests have already been carried out to verify these detrimental effects and their origin. Their is needed to well understand the whole process of cryopreservatio...

Endothelial cell detachment may cause failure of blood vessel and corneal cryopreservation, and thus successful cryopreservation of endothelial cells is regarded to be the first step to optimize cryopreservation of endothelial cells containing tissues. In this study, the pre-determined biophysical parameters were incorporated into the model for intracellular ice formation (IIF) and the growth o...

ABSTRACT Vriesea reitzii is an endemic bromeliad from the Atlantic Rainforest. The objective of this research was to evaluate cryopreservation using method direct immersion its seeds, collected capsules at 120, 135, and 150 days after anthesis (DAA). water content determined before cryopreservation, while germination percentage, speed index (GSI), total soluble carbohydrates were quantified cry...

Oocyte cryopreservation for fertility preservation, in both medical and elective situations, has significantly increased as freezing technology has improved. Slow freezing techniques demonstrated ∼ 50-80% survival of mature oocytes, however vitrification with ∼ 97% survival has become the preferred method for oocyte cryopreservation around the world. Our work investigated the effect of transpor...