OBJECTIVE To evaluate the effect of magnetic-activated cell sorting in cryopreservation-thawing protocols on sperm motility and cryosurvival rate. DESIGN Prospective-controlled study. SETTING Andrology department at a university-based medical institution. PATIENT(S) Ten healthy volunteer sperm donors. INTERVENTION(S) Sperm populations were separated using annexin-V magnetic-activated ce...

Low-density lipoprotein (LDL) extracted from hen egg yolk has recently been considered to be superior to whole egg yolk in sperm cryopreservation of various animal species. Meanwhile, there was a notion that high-density lipoprotein (HDL) in egg yolk may have a negative effect on post-thaw survival. The role of LDL and HDL in sperm cryopreservation of rhesus monkeys has not been explored. The p...

OBJECTIVES To review a large experience with sperm cryopreservation in patients with testicular cancer and determine the effect of clinical stage and tumor histologic features on semen quality. METHODS The prefreeze and post-thaw sperm quality of 157 patients with testicular cancer was compared with that of 50 normal donors. The impact of tumor stage and histologic features (pure seminoma, pu...

Cryopreservation induces injuries to fish spermatozoa that in turn affect sperm quality in terms of fertilization ability, motility, DNA and protein integrity and larval survival. To reduce the loss of sperm quality due to freezing-thawing, it is necessary to improve these procedures. In the present study we investigated the ability of two antifreeze proteins (AFPI and AFPIII) to reduce the los...

Cryopreservation of spermatozoa from endangered species is a valuable tool for genetic management. Previous studies showed the feasibility of cryopreservation of spermatozoa from various endangered gazelles but have also revealed difficulties with available protocols for semen freezing in Cuvier's gazelle (Gazella cuvieri). Experiments were carried out to investigate the effect of (a) 5% or 20%...

Ex situ conservation of genetic material from livestock and fish through cryopreservation is an important strategy to conserve genetic diversity in these species. Conservation strategies benefit from advances in cryopreservation and reproductive technologies. Choice of type of genetic material to be preserved for different species highly depends on objectives, technical feasibility (e.g., colle...

Cryopreservation of human sperm, now generally required in donor insemination programs, adversely affects the sperm in terms of standard sperm evaluation parameters and fertilizing ability. The freeze-thaw process appears to produce sublethal damage that appears only after a delay. The authors hypothesized that cryopreservation enhanced peroxidation of sperm membrane lipids, based on previous s...

BACKGROUND An approach consisting of elective cryopreservation of all embryos has been proposed for patients at risk of ovarian hyperstimulation syndrome (OHSS). Although elective cryopreservation can prevent pregnancy-induced late OHSS, it cannot prevent early OHSS. Early OHSS is reported to have been complicated with thromboembolism. The study was carried out to assess the efficacy with which...

This seventh statement of the ESHRE Task Force on Ethics and Law considers ethical questions and specific dilemmas surrounding the cryopreservation of gametes and reproductive tissue. This is of particular relevance with advancing techniques for cryopreservation and the desire of many individuals to preserve fertility after cancer, chronic illness, iatrogenic complications of treatment or simpl...