Large culture collections of microalgae and cyanobacteria such as the Coimbra Collection of Algae (ACOI) hold unialgal cultures consisting of a population of cells/colonies of a certain species. These cultures are usually non-axenic, as other organisms such as bacteria and microfungi are also present in culture due to co-isolation. Attention has been recently given to partner organisms since st...

BACKGROUND Optimal cryopreservation permits the long-term storage of living cells or tissues that may have potential clinical applications. Unfortunately, there are no successful studies on the long-term preservation of adipose aspirates for possible autologous fat grafting. OBJECTIVE The purpose of the current study was (1) to test our hypothesis that adipose aspirates obtained from conventi...

objective(s):althoughroutinely applied in assisted reproductive technology, human sperm cryopreservation is not a completely successful procedure. adverse effects of cryopreservation on the fertilization capacity, motility, morphology, and viability of spermatozoa have been proven; cryopreservation has also shown a role in sperm dna fragmentation and infertility. the post-thaw survival of sperm...

BACKGROUND An effect of cryopreservation on human sperm is sublethal cryodamage, in which cell viability post-thaw is lost more rapidly at later times than in fresh cells. OBJECTIVE This study examined whether the addition of an antioxidant to cryopreservation medium could improve the post-thaw parameters and evaluation of sperm chromatin quality of cryopreserved human spermatozoa from men wi...

Cryopreservation injuries involve nuclear DNA damage. A protocol for cryopreserving and isolating adipocyte nuclei is proposed. Adipose tissue samples were directly analyzed (NoCRYO-0h), or stored at -196°C for 7 days without 10% dimethyl sulfoxide (DMSO) (CRYO-WO-DMSO) or with DMSO (CRYO-W-DMSO). To determine the effect of DMSO on cryopreservation treatment, adipose tissue samples were stored ...

BACKGROUND Endothelial progenitor cells (EPC) are markers of endothelial injury and may serve as a surrogate marker for vascular repair in interventional clinical trials. Objectives of this study were to modify a method of isolation of peripheral blood mononuclear cells (PBMC) and enumeration of EPC and mature endothelial cells (EC) from peripheral blood and to evaluate influence of cryopreserv...

Backgroud: Oocyte cryopreservation is critical in assisted reproduction techniques. However, the effect of cryopreservation on the oocyte has not been widely explored yet. Currently a thorough understanding of oocyte cryobiology is urgently required due to its far lag behind the forces propelling the clinical application of oocyte cryopreservation. Methods: In this study, we used two different ...

in the present study, the fertilizing potential of semen recovered from slaughtered bulls epididymis was evaluated after cryopreservation, by conventional techniques and flow cytometry methods. the cauda epididymal was dissected and sperm were recovered and evaluated for volume, sperm concentration, and membrane and acrosome integrity using a flow cytometer. sperm fertility potential was tested...

Aim: The objective of this descriptive study was to evaluate the attitudes towards oocyte cryopreservation among Turkish nursing and midwifery students. Method: sample consisted 342 female students in departments two universities Istanbul. A questionnaire about their socio-demographic characteristics, knowledge views applied online volunteer Results: average age participants 20.96±1.8. wanted h...

improvements on embryo micromanipulation techniques led to the use of embryo biopsy in commercial embryo transfer programs for genetic analysis of preimplantation bovine embryos. the aim of this study was to evaluate the quality of bovine blastocyst derived from embryos biopsied at different precompacted morulae stages by assessment of cryosurvivability of the resulting blastocysts. the in vitr...