The incidence of CIA associated with regimens involving cyclophosphamide or anthracyclines ranges from 53-89%. And the incidence of amenorrhea reported from the recently presented BCIRG-01 trial comparing TAC; (Docetaxel, Doxorubicin, and cyclophosphamide) and FAC; (5-FU, Doxorubicin, and Cyclophosphamide) in early-stage breast cancer was 32.8% in patients receiving FAC. However, this trial was...

Gene therapy using the prodrug-activating enzyme P450 2B6 has shown substantial promise in preclinical and initial clinical studies with the P450 prodrugs cyclophosphamide and ifosfamide. We sought to optimize this therapy using the canine P450 enzyme 2B11, which activates cyclophosphamide and ifosfamide with Km of 80 to 160 micromol/L, approximately 10- to 20-fold lower than the Km of P450 2B6...

BACKGROUND 12 months of oral cyclophosphamide has been shown to alter the progression of scleroderma-related interstitial lung disease when compared with placebo. However, toxicity was a concern and without continued treatment the efficacy disappeared by 24 months. We hypothesised that a 2 year course of mycophenolate mofetil would be safer, better tolerated, and produce longer lasting improvem...

Cyclophosphamide is a widely used antitumor drug, with induced adverse effects, such as intestinal mucosal injury and immunosuppression. Norcantharidin possesses anticancer activity through enhancement of immunity. We investigated the role norcantharidin in cyclophosphamide-induced Mice were treated cyclophosphamide, exposed to norcantharidin. Enzyme-linked-immunosorbent serologic assay was per...

In the design of sequential high-dose chemotherapy regimens, the selection of antitumor alkylating agents to be included in each intensification and the interval between the intensifications are critical to the design of the therapy. The tumor cell survival assay and tumor growth delay assay using the murine EMT-6 mammary carcinoma were used as a solid tumor model in which to address these issu...

Influence of cyclophosphamide treatment (40. mg/kg, ip on alternate days for 5 times) was assessed on 1 m mol BaCl2-induced in vitro rhythmic contractions in isolated rat vas deferens. The mean frequency (contractions per min) and amplitude (g tension) of rhythmic contractions were significantly decreased in the tissues isolated from cyclophosphamide-treated rats as compared to the control tiss...

Cytotoxic potencies of cyclophosphamide, 4-ketocyclophosphamide, carboxyphosphamide, mechlorethamine (HN2), bis(2-chloroethyl)amine (nor-HN2), chlorambucil, acrolein, and cyclophosphamide metabolites generated in vivo and in vitro were determined via bioassay. Our bioassay procedure was to incubate the potential cytotoxic agent with Walker 256 ascites cells in vitro, inject these cells into hos...

Background: Cyclophosphamid as an anti-neoplastic medicine, is classified as a derivative of mustard nitrogen, which passes through brain- blood -barrier and it is known as causing significant poisoning affects with high stoppage effects on body. Through attaching two DNA chains of cell nucleus and breaking inter/intra-chain connection and inhibition RNA synthesis Cyclophosphamide causes high t...