The aim of this study was to investigate the therapeutic effects of propolis for the toxicity of cypermethrin (CYP) on histopathological changes in tissues of rainbow trout (Oncorhynchus mykiss). CYP is one of the most toxic pyrethroids highly for the aquatic organisms. The fish were exposed to three sublethal concentrations of CYP (0.0041, 0.0082 and 0.0123 ppm). In addition, different concent...

Xenobiotics, including drugs, can influence cytochrome P450 (CYP) activity by upregulating the transcription of CYP genes. To minimize potential drug interactions, it is important to ascertain whether a compound will be an inducer of CYP enzymes early in the development of new therapeutic agents. In vivo and in vitro studies are reported that demonstrate the use of liver and intestinal slices a...

Mifepristone (RU486), an 11beta-substituted nor-steroid containing a 17alpha-1-propynyl group used clinically as an antiprogestin agent for medical abortions, was demonstrated to be a selective mechanism-based inactivator of human cytochrome P-450-3A4 (CYP-3A4). The loss of testosterone 6beta-hydroxylation activity was time- and concentration-dependent as well as requiring metabolism of mifepri...

Eriocheir japonicus, fresh-water crabs inhabiting rivers and estuaries in Japan, were investigated for cytochrome P450 (CYP)-dependent drug-metabolizing enzyme activities to see if these activities reflect the river pollution gradient. From the laboratory dose-response experiments, we found that the polycyclic aromatic hydrocarbon (PAH) 3-methylcholanthrene induced total CYP contents, ethoxycou...

Triclosan (TCS) is a widespread antimicrobial agent with many adverse health risks. Its hepatoxicity invariably points to the activation of constitutive androstane receptor (CAR), which regulates cytochrome P450 (CYP) genes that are critical for oxidative metabolism. Here, we provide theoretical and experimental evidences showing metabolic TCS frequently occurs through aromatic hydroxylation in...

Activators of soluble guanylyl cyclase (sGC) interact directly with its prosthetic heme group, enhancing the enzyme responsiveness in pathological conditions. This study aimed to evaluate the effects of the sGC activator BAY 58-2667 on voiding dysfunction, protein expressions of α1 and β1 sGC subunits and cGMP levels in the bladder tissues after cyclophosphamide (CYP) exposure. Female C57BL/6 m...

BACKGROUND P450 fatty acid decarboxylases represented by the unusual CYP152 peroxygenase family member OleTJE have been receiving great attention recently since these P450 enzymes are able to catalyze the simple and direct production of 1-alkenes for potential applications in biofuels and biomaterials. To gain more mechanistic insights, broader substrate spectra, and improved decarboxylative ac...

Address for correspondence Mr. Mohammad Asif, Department of Pharmacy, GRD (P.G) Institute of Management and Technology, 214-Rajpur Road, Dehradun (U.K), 248009, India. Phone No: +91-9897088910 E-mail: [email protected] ABSTRACT Purpose: In the present study, antitubercular activities and in silico physicochemical toxicities and bioactivity profile of some 3-chloro-6-arylpyridazines (3a-d) and ...

Although various in vitro assays have been developed to evaluate the cytochrome P450 (CYP)-inducing potential of drug candidates, there is a continuing need for the development of a reliable model in drug discovery. The objective of the present study was to compare CYP induction by chemicals in HepG2 cells with Huh7, NKNT-3, and reverted NKNT-3 cells. HepG2 cells showed more similarity to human...

abstract   the mutagenic and immunosuppressory effects of cyclophosphamide (cyp) are still the primary limitation to wider application for treating a variety of human malignancies. on the one hand, cyp treatment predisposes transplant recipients and cancer patients to risk of bacterial, fungal, and viral infections, in the other word the, lipopolysaccharide (lps), an endotoxin found in cell wal...